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Review
. 2015 Sep;16(9):568-76.
doi: 10.1038/nrm4014. Epub 2015 Jun 17.

Bricks and blueprints: methods and standards for DNA assembly

Arturo Casini  1 , Marko Storch  1 , Geoffrey S Baldwin  1 , Tom Ellis  2
Affiliations
Free article
Review

Bricks and blueprints: methods and standards for DNA assembly

Arturo Casini et al. Nat Rev Mol Cell Biol. 2015 Sep.
Free article

Abstract

DNA assembly is a key part of constructing gene expression systems and even whole chromosomes. In the past decade, a plethora of powerful new DNA assembly methods - including Gibson Assembly, Golden Gate and ligase cycling reaction (LCR) - have been developed. In this Innovation article, we discuss these methods as well as standards such as the modular cloning (MoClo) system, GoldenBraid, modular overlap-directed assembly with linkers (MODAL) and PaperClip, which have been developed to facilitate a streamlined assembly workflow, to aid the exchange of material between research groups and to create modular reusable DNA parts.

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References

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