Site-Specific Glycosylation Profiling Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS)
- PMID: 26082237
- DOI: 10.1007/978-1-4939-2760-9_27
Site-Specific Glycosylation Profiling Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS)
Abstract
A method for comprehensive glycoprotein characterization via glycopeptide generation and analysis is presented. Parallel analysis of a deglycosylated sample and the use of buffered solvent simplify the identification of peaks comprising the glycopattern of a given peptide.
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