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. 2015:1321:407-15.
doi: 10.1007/978-1-4939-2760-9_27.

Site-Specific Glycosylation Profiling Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS)

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Site-Specific Glycosylation Profiling Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS)

Clemens Gruber et al. Methods Mol Biol. 2015.

Abstract

A method for comprehensive glycoprotein characterization via glycopeptide generation and analysis is presented. Parallel analysis of a deglycosylated sample and the use of buffered solvent simplify the identification of peaks comprising the glycopattern of a given peptide.

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