Guardians of the Gut - Murine Intestinal Macrophages and Dendritic Cells

Abstract

Intestinal mononuclear phagocytes find themselves in a unique environment, most prominently characterized by its constant exposure to commensal microbiota and food antigens. This anatomic setting has resulted in a number of specializations of the intestinal mononuclear phagocyte compartment that collectively contribute the unique steady state immune landscape of the healthy gut, including homeostatic innate lymphoid cells, B, and T cell compartments. As in other organs, macrophages and dendritic cells (DCs) orchestrate in addition the immune defense against pathogens, both in lymph nodes and mucosa-associated lymphoid tissue. Here, we will discuss origins and functions of intestinal DCs and macrophages and their respective subsets, focusing largely on the mouse and cells residing in the lamina propria.

Keywords: IBD; dendritic cells; gut; homeostasis; inflammation; macrophages.

Figure 1

CyTOF analysis of CD45⁺ cells from murine colon. Cells were isolated from colon of 6–9 weeks old WT female C57Bl/6 mice and stained with a panel of 26 cell surface markers. The results were gated for live, single, CD45⁺ cells. Bh-SNE analysis and clustering were performed by Accense (http://www.cellaccense.com/) and the results were processed by GIMP. Colors indicate high levels of the following markers: green – TCRβ, CD3e (T cells), Orange – B220 (B cells), light blue – Ly6G (granulocytes), pink –Ly6C (monocytes), purple – CD64, F4/80 (macrophages), blue – clustered by Accence, different DC populations, gray – non-identified or non-specific cells. Red populations in zoom-in black squares indicate high levels of the marker written. Representative of at least four separate, independent experiments.