Single Molecules, Cells, and Super-Resolution Optics (Nobel Lecture)

Abstract

The resolution of a microscope is determined by the diffraction limit in classical microscopy, whereby objects that are separated by half a wavelength can no longer be visually separated. To go below the diffraction limit required several tricks and discoveries. In his Nobel Lecture, E. Betzig describes the developments that have led to modern super high-resolution microscopy.

Keywords: PALM; microscopy; near-field; super-resolution.