Distribution of Peripheral Lymphocyte Populations in Primary Sjögren's Syndrome Patients

Abstract

Purpose of this study was to evaluate the lymphocyte populations' distribution changes in peripheral blood of patients with primary Sjögren's syndrome (pSS). Lymphocyte populations' distribution changes in peripheral blood of pSS patients were investigated in 52 patients with pSS and in 28 healthy controls by flow cytometry. We found decreased absolute count of CD3(+) T cell population in pSS patients. Analysis of CD4(+) T cell population showed significant proportion and absolute count differences in pSS patient's blood with SSA/SSB antibodies (Abs) in comparison to controls. No significant differences were observed analyzing CD4(+) and CD8(+) Treg subpopulation. Proportion and absolute counts of Th17 cells were significantly lower in pSS patient's blood. Absolute counts of CD8(+) T cells were significantly lower in pSS patients in comparison to controls and also impaired proportion and absolute counts of CD8(+) subpopulations according to CD27(+) and CD57(+) were observed. Absolute counts of NKT and NK cells were decreased in pSS with Abs. B cells proportion was increased only in blood of pSS with Abs. Lymphocyte distribution impairment can be due to genetically determined lymphopenia or lymphocyte migration from periphery to inflammatory sites or/and increased susceptibility to apoptosis.

Figure 1

Representative dot plots. CD8^high lymphocytes in the CD8⁺ subset were determined in the flow cytometric SSC/CD8-PerCP dot plot and percentages of CD8^highCD57⁻CD27⁺ lymphocytes were determined in gated CD8^high subset in the flow cytometric CD57-FITC/CD27-APC dot plot.

Figure 2

Representative dot plots. CD4⁺IL-17A⁺ positive cells were determined on CD4-PerCP versus IL-17A-APC dot plot by gating on lymphocyte on the forward-scatter versus side-scatter dot plot.