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. 2015:559:17-26.
doi: 10.1016/bs.mie.2014.11.004. Epub 2015 Apr 15.

Affinity Purification of a Recombinant Protein Expressed as a Fusion with the Maltose-Binding Protein (MBP) Tag

Krisna C Duong-Ly  1 , Sandra B Gabelli  2
Affiliations

Affinity Purification of a Recombinant Protein Expressed as a Fusion with the Maltose-Binding Protein (MBP) Tag

Krisna C Duong-Ly et al. Methods Enzymol. 2015.

Abstract

Expression of fusion proteins such as MBP fusions can be used as a way to improve the solubility of the expressed protein in E. coli (Fox and Waugh, 2003; Nallamsetty et al., 2005; Nallamsetty and Waugh, 2006) and as a way to introduce an affinity purification tag. The protocol that follows was designed by the authors as a first step in the purification of a recombinant protein fused with MBP, using fast protein liquid chromatography (FPLC). Cells should have been thawed, resuspended in binding buffer, and lysed by sonication or microfluidization before mixing with the amylose resin or loading on the column. Slight modifications to this protocol may be made to accommodate both the protein of interest and the availability of equipment.

Keywords: Affinity chromatography; Affinity purification; Fast protein liquid chromatography (FPLC); Fusion protein; MBP fusion; Maltose-binding protein (MBP); Recombinant protein.

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Figures

Figure 1
Figure 1
Chromatograph of a purification of an MBP-tagged fusion protein using amylose beads.
Figure 2
Figure 2
Flowchart of the complete protocol, including preparation.
Figure 3
Figure 3
Flowchart of Step 1.
Figure 4
Figure 4
Flowchart of Steps 2 and 3.
Figure 5
Figure 5
Flowchart of Step 4.
See this image and copyright information in PMC

References

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