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. 2015 Nov;77(11):1371-7.
doi: 10.1292/jvms.14-0657. Epub 2015 Jun 21.

Epidemiology and genetic characterization of BVDV, BHV-1, BHV-4, BHV-5 and Brucella spp. infections in cattle in Turkey

Muhammet Eren Aslan  1 , Ahmet Kursat Azkur, Serkal Gazyagci
Affiliations

Epidemiology and genetic characterization of BVDV, BHV-1, BHV-4, BHV-5 and Brucella spp. infections in cattle in Turkey

Muhammet Eren Aslan et al. J Vet Med Sci. 2015 Nov.

Abstract

The aim of the study was to determine the epidemiological data of bovine viral diarrhea virus (BVDV), bovine herpesvirus-1 (BHV-1), bovine herpesvirus-4 (BHV-4), bovine herpesvirus-5 (BHV-5) and Brucella-associated cattle that were previously reported to have abortion and infertility problems in Ankara, Corum, Kirikkale and Yozgat provinces, Turkey. Whole blood and sera samples were obtained from 656 cattle, and antibodies against Brucella spp. were detected in 45 (6.86%) and 41 (6.25%) animals by Rose Bengal plate and serum tube agglutination tests, respectively. The seropositivity rates against BVDV, BHV-1 and BHV-4 were 70.89%, 41.3% and 28.78%, respectively. RT-PCR and PCR were performed to detect RNA and DNA viruses in blood samples, respectively. The BVDV 5'-untranslated region and BHV-1 gB gene detected in this study were phylogenetically analyzed. The BVDV strains analyzed in this study were closely related to those previously reported from Turkey. The nucleotide sequence from the BHV-1 strain detected in this study is the first nucleotide sequence of BHV-1 circulating in this area of Turkey deposited in the GenBank. The presence of Brucella spp. and prevalence of BHV-1, BHV-4 and BVDV in cattle should be further investigated throughout these regions.

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Figures

Fig. 1.
Fig. 1.
The seropositivity rates of BHV-1, BHV-4 and BVDV infections in cattle sera. A, single-, double- and triple-positive samples; B, samples seronegative for BVDV, BHV-1 and BHV-4.
Fig. 2.
Fig. 2.
BVDV phylogenetic analysis. The phylogenetic maximum likelihood tree for the 5′-UTR of the pestivirus genome was generated by using the MEGA 6 software. Sequences generated in this study were as follows: 119-KF425303, 74-KF434627, 60-KF434625, 62-KF434626, 32-KF434623, 24-KF434622, 22-KF434621, 20-KF434620, 14-KF434618, 16-KF434619, 12-KF425300, 45-KF425299, 413-KF425302, 414-KF425301, 90-KF434630, 84-KF434629, 81-KF434628 and 42-KF434624. Sequences not generated in this study were derived from GenBank and were as follows: HQ393488, HQ393489, EU716142, EU716128, DQ973179, EU716151, AF298062, EU716126, AF298069, AF298066, FJ493485, AF299317, EU716118, DQ973172 and EU716125 for BVDV-1; EU542421 and AB042676 for BVDV-2; AM418428, FJ493487, GU979820.1, GU979819.1 and GU979818.1 for BDV; and AJ605590 for CSFV.
Fig. 3.
Fig. 3.
The phylogenetic tree for the gB gene of BHV-1. The phylogenetic tree was inferred using the MEGA 6 and Clustal omega softwares. Sequences (EF175730.1, KJ652520.1, KJ652518.1, KF584168.1, KC479146.1, KF601566.1, KF601565.1, KC479149.1, KC479143.1, JF920419.1, DQ006854.1, KF734616.1, KC682098.1, JN106444.1, AY330349.1, EU272475.1, JF920420.1, JF920418.1, EU523744.1, AF258347.1, DQ006857.1, EF624475.1, KJ652519.1 and JX898220.1) were not generated by this study, but were derived from GenBank for the KF716130.1 sequence.
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