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. 2015:2015:161579.
doi: 10.1155/2015/161579. Epub 2015 May 25.

T, B, and NKT Cells in Systemic Inflammation in Obstructive Sleep Apnoea

Joanna Domagała-Kulawik  1 , Iwona Osińska  1 , Aleksandra Piechuta  1 , Piotr Bielicki  1 , Tomasz Skirecki  2
Affiliations

T, B, and NKT Cells in Systemic Inflammation in Obstructive Sleep Apnoea

Joanna Domagała-Kulawik et al. Mediators Inflamm. 2015.

Abstract

Background: Obstructive sleep apnoea syndrome (OSAS) brings risk of serious complications. The study objective was to assess elements of the cellular immune response in the course of OSAS.

Methods: Peripheral blood (PB) lymphocytes: T, B, NK, NKT-like, Th, Tc, and HLA DR+ T cells were evaluated by flow cytometry of 48 OSA patients; the concentration of adiponectin, interleukin 1β, and TNFα was measured by ELISA method. The OSA complication score was developed and used for statistical analysis.

Results: The proportion of B cells and Th/Tc ratio were significantly lower in the BP of OSA patients when compared with control subjects (median 7.9 versus 10.9%, 0.9 versus 1.5, p < 0.05). The proportion of Tc, NK, NKT-like, and HLADR positive T cells were elevated in OSA patients when compared with healthy subjects (36.4 versus 26.8, 15.5 versus 8.5, 5.7 versus 3.0, and 8.4 versus 4.5%, p < 0.05, resp.) and were more pronounced in patients with metabolic syndrome. The grade of OSA complication score correlated with systemic inflammation markers and the proportion of B cells. The value of adiponectin/BMI ratio correlated significantly with SpO2 (r = 0.31, p < 0.05), CRP (r = -0.35, p < 0.05), TNFα concentration (r = -0.36, p < 0.05), and proportion of B cells (r = 0.32, p < 0.05).

Conclusion: Lymphocytes B, Tc, NK, NKT-like, and adiponectin are involved in systemic immune response in OSA patients possibly predisposing them to cardiovascular and metabolic complications.

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Figures

Figure 1
Figure 1
OSA complication score and the number of patients distribution in each group. The description of score was included in the main text.
Figure 2
Figure 2
Median concentration of adiponectin in peripheral blood of OSA patients and healthy subjects. Plots are the 10th, 25th, 75th, and 90th percentiles as vertical boxes with error bars. 1: all OSA patients; 2: healthy subjects; 3: OSA patients with BMI < 25; 4: obese OSA patients; 5: no obese OSA patients; 6: OSA patients with metabolic syndrome; 7: OSA patients without metabolic syndrome. There was significant difference between groups 3 and 4, 6, and 7 in Kruskal-Wallis test (p < 0.05).
Figure 3
Figure 3
Significant correlation between oxygen desaturation index (ODI) and the number of NKT-like cells in the peripheral blood of OSA patients (r = 0.4, p < 0.05).
See this image and copyright information in PMC

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