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. 2015 May;11(Suppl 1):S166-72.
doi: 10.4103/0973-1296.157722.

Development of response surface methodology for optimization of extraction parameters and quantitative estimation of embelin from Embelia ribes Burm by high performance liquid chromatography

Md Shamsir Alam  1 , Zoheir A Damanhouri  2 , Aftab Ahmad  3 , Lubna Abidin  1 , Mohd Amir  1 , Mohd Aqil  4 , Shah Alam Khan  5 , Mohd Mujeeb  1
Affiliations

Development of response surface methodology for optimization of extraction parameters and quantitative estimation of embelin from Embelia ribes Burm by high performance liquid chromatography

Md Shamsir Alam et al. Pharmacogn Mag. 2015 May.

Abstract

Background: Embelia ribes Burm is widely used medicinal plant for the treatment of different types of disorders in the Indian traditional systems of medicine.

Objective: The present work was aimed to optimize the extraction parameters of embelin from E. ribes fruits and also to quantify embelin content in different extracts of the plant.

Materials and methods: Optimization of extraction parameters such as solvent: drug ratio, temperature and time were carried out by response surface methodology (RSM). Quantitative estimation of embelin in different extracts of E. ribes fruits was done through high performance liquid chromatography.

Results: The optimal conditions determined for extraction of embelin through RSM were; extraction time (27.50 min), extraction temperature 45°C and solvent: drug ratio (8:1). Under the optimized conditions, the embelin yield (32.71%) was equitable to the expected yield (31.07%, P > 0.05). These results showed that the developed model is satisfactory and suitable for the extraction process of embelin. The analysis of variance showed a high goodness of model fit and the accomplishment of the RSM method for improving embelin extraction from the fruits of E. ribes.

Conclusion: It is concluded that this may be a useful method for the extraction and quantitative estimation of embelin from the fruits of E. ribes.

Keywords: Embelia ribes; response surface methodology; reverse phase-high performance liquid chromatography; ultrasonic-assisted extraction.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Chemical structure of embelin
Figure 2
Figure 2
Calibration curve of standard embelin with respect to peak area
Figure 3
Figure 3
Effect of extraction temperature on embelin yield
Figure 4
Figure 4
Effect of extraction time on embelin yield
Figure 5
Figure 5
Effect of solvent: drug ratio on embelin yield
Figure 6
Figure 6
Dimensional response surface plot for embelin extraction showing interactive effects of temperature and time
Figure 7
Figure 7
Dimensional response surface plot for embelin extraction showing interactive effects of temperature and drug: solvent ratio
Figure 8
Figure 8
Dimensional response surface plot for embelin extraction showing interactive effects of time and drug: solvent ratio
Figure 9
Figure 9
Contour Plot of temperature and time
Figure 10
Figure 10
Contour plot of temperature and drug: Solvent ratio
Figure 11
Figure 11
Contour Plot of time and drug: solvent ratio
Figure 12
Figure 12
High performance liquid chromatography chromatogram of standard embelin (80ìg/mL). Retention time - 3.891 min
Figure 13
Figure 13
High performance liquid chromatography chromatogram of E ribes fruit extract. Retention time- 3.853 minutes
See this image and copyright information in PMC

References

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