Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2015 Aug 15;25(16):3079-86.
doi: 10.1016/j.bmcl.2015.06.023. Epub 2015 Jun 12.

Hide and seek: Identification and confirmation of small molecule protein targets

Affiliations
Review

Hide and seek: Identification and confirmation of small molecule protein targets

Andrei Ursu et al. Bioorg Med Chem Lett. .

Abstract

Target identification and confirmation for small molecules is often the rate limiting step in drug discovery. A robust method to identify proteins addressed by small molecules is affinity chromatography using chemical probes. These usually consist of the compound of interest equipped with a linker molecule and a proper tag. Recently, methods emerged that allow the identification of protein targets without prior functionalization of the small molecule of interest. The digest offers an update on the newest developments in the area of target identification with special focus on confirmation techniques.

Keywords: Affinity chromatography; Chemical probes; Linker; Target confirmation; Target identification.

PubMed Disclaimer

Publication types

MeSH terms

LinkOut - more resources