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. 1989 Dec;16(5-6):429-32.
doi: 10.1007/BF00340722.

A petite positive strain of Kluyveromyces lactis has a 300 kb deletion in the rDNA cluster

R Maleszka  1 , G D Clark-Walker
Affiliations

A petite positive strain of Kluyveromyces lactis has a 300 kb deletion in the rDNA cluster

R Maleszka et al. Curr Genet. 1989 Dec.

Abstract

By employing Pulsed Field Gel Electrophoresis we have shown that the chromosomal DNA pattern of a petite-positive strain of Kluyveromyces lactis, designated KF4, differs from the karyotype of the reference strain. Digestion with SfiI and hybridization with an rDNA probe, demonstrate that a novel chromosomal band in KF4 results from a deletion of 35-40 rDNA cistrons from the rDNA cluster. The significance of this finding to possible alterations in cell physiology and the ability to generate "petite" mutants is discussed.

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References

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