doi: 10.1016/s0021-9673(00)81078-x.
Determination of indomethacin in serum and urine by electron-capture gas-liquid chromatography
- PMID: 2612
- DOI: 10.1016/s0021-9673(00)81078-x
Item in Clipboard
Determination of indomethacin in serum and urine by electron-capture gas-liquid chromatography
J Chromatogr.
.
Abstract
A specific and sensitive method for the quantitative determination of indomethacin in serum and urine is described. The drug is extracted at pH 5.0 with 1,2-dichloroethane and a portion of the organic extract is concentrated and made to react with diazoethane in diethyl ether. The ethyl ester derivative is analyzed by electron-capture gas-liquid chromatography, quantitation being achieved by comparison of peak areas for samples and standards, which are prepared in serum or urine and treated in the same manner as the samples. The limit of sensitivity is 50 ng/ml and the relative standard derivation for repeat determinations on the same sample is about 3%.
Similar articles
-
Quantitative gas-liquid chromatographic method for the determination of indomethacin in biological fluids.J Chromatogr. 1978 Jun 1;153(1):189-94. doi: 10.1016/s0021-9673(00)89871-4. J Chromatogr. 1978. PMID: 659554
-
High-pressure liquid chromatographic method with postcolumn, in-line hydrolysis and fluorometric detection for indomethacin in biological fluids.J Pharm Sci. 1981 Apr;70(4):458-9. doi: 10.1002/jps.2600700431. J Pharm Sci. 1981. PMID: 7229968
-
Sensitive fused-silica capillary gas chromatographic assay using electron-capture detection for indomethacin in ovine fetal fluids.J Chromatogr B Biomed Appl. 1995 Dec 1;674(1):65-75. doi: 10.1016/0378-4347(95)00291-x. J Chromatogr B Biomed Appl. 1995. PMID: 8749253
-
Gas chromatographic determination of indomethacin and its O-desmethylated metabolite in human plasma and urine.J Chromatogr. 1983 Oct 14;277:368-73. doi: 10.1016/s0378-4347(00)84860-4. J Chromatogr. 1983. PMID: 6643623 No abstract available.
-
Determination of indomethacin in serum by an extractive alkylation technique and gas-liquid chromatography.J Chromatogr. 1978 Jun 1;153(1):195-202. doi: 10.1016/s0021-9673(00)89872-6. J Chromatogr. 1978. PMID: 659555
Cited by
-
Clinical Pharmacokinetics of indomethacin.Clin Pharmacokinet. 1981 Jul-Aug;6(4):245-58. doi: 10.2165/00003088-198106040-00001. Clin Pharmacokinet. 1981. PMID: 7249487
-
Indomethacin treatment in small versus large premature infants with ductus arteriosus. Comparison of plasma indomethacin concentration and clinical response.Br Heart J. 1983 Jul;50(1):27-30. doi: 10.1136/hrt.50.1.27. Br Heart J. 1983. PMID: 6860508 Free PMC article.
-
Plasma indomethacin assay by reversed-phase ion pair high pressure liquid chromatography.Pharm Weekbl Sci. 1983 Dec 16;5(6):313-8. doi: 10.1007/BF02074861. Pharm Weekbl Sci. 1983. PMID: 6664824
-
Development of Physiologically Based Pharmacokinetic/Pharmacodynamic Model for Indomethacin Disposition in Pregnancy.PLoS One. 2015 Oct 2;10(10):e0139762. doi: 10.1371/journal.pone.0139762. eCollection 2015. PLoS One. 2015. PMID: 26431339 Free PMC article.