Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Jun 30:15:60.
doi: 10.1186/s12872-015-0041-1.

Correlation of single nucleotide polymorphisms in the pregnancy-associated plasma protein-A gene with carotid plaques

Shiming Zhou  1 , Min Cui  2 , Zegang Yin  3 , Rui Li  4 , Jie Zhu  5 , Huadong Zhou  6
Affiliations

Correlation of single nucleotide polymorphisms in the pregnancy-associated plasma protein-A gene with carotid plaques

Shiming Zhou et al. BMC Cardiovasc Disord. .

Abstract

Background: Pregnancy-associated plasma protein A (PAPP-A) is abundantly expressed in carotid plaques. This study investigated the association between single nucleotide polymorphisms (SNPs) of PAPP-A and the presence of carotid plaques.

Methods: A total of 408 patients with carotid plaques and 493 controls were included in the study. All subjects were Southern Chinese Han. Carotid plaques were analyzed by computer tomography angiography. PAPP-A SNPs were identified by ligase detection reaction-polymerase chain reaction analysis. The PAPP-A genotypes rs3747823, rs7020782, and rs13290387 were analyzed.

Results: The rs7020782 C allele genotype correlated with an increased risk of developing carotid plaques under the dominant, recessive, and additive models (adjusted odds ratios: 2.60, 2.36, and 3.48, respectively; P ≤ 0.001). Only C allele-carrying genotypes correlated with a significantly increased risk of carotid plaque based on studies stratified by age and sex under the dominant model. rs7020782 remained significantly associated with the risk of carotid plaque calcification after adjusting for age and potential confounders (adjusted odds ratio, 1.89; 95 % confidence interval, 1.17-3.08; P = 0.010).

Conclusions: This study found, for the first time, that the A˃C variation of rs7020782 might be an independent risk factor for carotid plaque development and calcification. The determination of such genotypes could provide a new tool for identifying individuals at high risk for carotid atherosclerosis.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Dominant model of rs7020782 C allele in subjects with calcified plaque and non-calcified plaque. Chi-Squared test for categorical values Pearson χ 2 = 6.665, P = 0.010 for genotype frequency. CP: calcified plaque NCP: non-calcified plaque. Adjusted for age, sex, systolic blood pressure, diastolic blood pressure, high density lipoprotein cholesterol, the risk of calcified plaque: odds ratio, 1.89, 95 % CI,1.17-3.08, P = 0.010
Fig. 2
Fig. 2
Stratified distribution of rs7020782 genotypes by sex in subjects with carotid plaque and controls. Adjusted for age, systolic blood pressure, diastolic blood pressure, high density lipoprotein cholesterol, the risk of carotid plaque under C allele dominant model in female subjects*: odds ratio, 2.08; 95 % CI, 1.38–3.14; P < 0.001, male subjects** : odds ratio, 3.14; 95 % CI, 2.05–4.81; P < 0.001
Fig. 3
Fig. 3
Stratified distribution of rs7020782 genotypes by age in subjects with carotid plaque and controls. Adjusted for sex, systolic blood pressure, diastolic blood pressure, high density lipoprotein cholesterol, the risk of carotid plaque under C allele dominant model in subjects aged ≤64 years *: odds ratio, 2.33; 95 % CI, 1.47–3.69; P < 0.001, those aged > 64 years** : odds ratio, 2.57; 95 % CI, 1.75–3.76; P < 0.001
See this image and copyright information in PMC

References

    1. Bayes-Genis A, Schwartz RS, Lewis DA, Overgaard MT, Christiansen M, Oxvig C, et al. Insulin-like growth factor binding protein-4 protease produced by smooth muscle cells increases in the coronary artery after angioplasty. Arteriosclerosis, Thrombosis & Vascular Biology. 2001;21(3):335–41. doi: 10.1161/01.ATV.21.3.335. - DOI - PubMed
    1. Boldt HB, Overgaard MT, Laursen LS, Weyer K, Sottrup-Jensen L, Oxvig C. Mutational analysis of the proteolytic domain of pregnancy-associated plasma protein-A (PAPP-A): classification as a metzincin. Biochemical Journal. 2001;358(Pt 2):359–67. doi: 10.1042/0264-6021:3580359. - DOI - PMC - PubMed
    1. Vallejo AN, Michel JJ, Bale LK, Lemster BH, Borghesi L, Conover CA. Resistance to age-dependent thymic atrophy in long-lived mice that are deficient in pregnancy-associated plasma protein A. P Natl Acad Sci USA. 2009;106(27):11252–7. doi: 10.1073/pnas.0807025106. - DOI - PMC - PubMed
    1. Lawrence JB, Oxvig C, Overgaard MT, Sottrup-Jensen L, Gleich GJ, Hays LG, et al. The insulin-like growth factor (IGF)-dependent IGF binding protein-4 protease secreted by human fibroblasts is pregnancy-associated plasma protein-A. P Natl Acad Sci USA. 1999;96(6):3149–53. doi: 10.1073/pnas.96.6.3149. - DOI - PMC - PubMed
    1. Bayes-Genis A, Conover CA, Overgaard MT, Bailey KR, Christiansen M, Holmes DR, Jr, et al. Pregnancy-associated plasma protein A as a marker of acute coronary syndromes. N Engl J Med. 2001;345(14):1022–9. doi: 10.1056/NEJMoa003147. - DOI - PubMed

Publication types

MeSH terms

Substances