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. 2015 Jul-Sep;7(3):282-93.
doi: 10.4103/0974-8490.157179.

Structural elucidation of chemical constituents from Benincasa hispida seeds and Carissa congesta roots by gas chromatography: Mass spectroscopy

Gaurav M Doshi  1 , Vivek V Nalawade  2 , Aaditi S Mukadam  3 , Pratip K Chaskar  2 , Sandeep P Zine  2 , Rakesh R Somani  4 , Hemant D Une  5
Affiliations

Structural elucidation of chemical constituents from Benincasa hispida seeds and Carissa congesta roots by gas chromatography: Mass spectroscopy

Gaurav M Doshi et al. Pharmacognosy Res. 2015 Jul-Sep.

Abstract

Background: Benincasa hispida (BH) and Carissa congesta (CC) are regarded as ethnopharmacological imperative plants in Asian countries.

Objective: Phytochemical screening of the extracts has shown the presence of steroids, flavonoids, saponins, glycosides, tannins, phenolic compounds, fixed oils, and fats in the BH and CC extracts. The presence of lupeol has been reported previously by us using high-performance thin-layer chromatography and high-performance liquid chromatography.

Materials and methods: Present research studies encompasses identification of chemical constituents in BH seeds and CC roots petroleum ether extracts by hyphenated technique such as gas chromatography-mass spectroscopy (MS) which when coupled gives a clear insight of constituents.

Results: The components were identified by matching mass spectra with MS libraries. There were 13 and 10 different compounds analyzed from CC and BH, respectively. The components present were Pentanoic acid, 5-hydroxy, 2,4-butylphenyl; n-Hexadecanoic acid (Palmitic acid); Sulfurous acid, 2-ethylhexylhepatdecyl ester; n-Tridecane; 6-methyltridecane; (9E, 12E)-9,12-Octadecadienyl chloride, Hexadecanoic acid, 3-(trimethylsilyl)-oxy] propyl ester; 9,12-Octadecadenoic acid, 2 hydroxy-1-(hyroxymethylethyl) ester; 9,12-Octadecadienoic acid, 2,3 dihydroxypropyl ester; n-Propyl-9,12-Octadecadienoate, Lupeol; Taraxasterol; 6a, 14a-Methanopicene, perhydro-12,4a, 61a, 9,9,12a-hepatmethyl-10-hydoxy and 9-Octadecene; 2-Isoprpenyl-5-methyl-6-hepten-1-ol; n-Hexadecanoic acid, 2-hyroxy-1-(hydroxymethyl) ethyl ether; Butyl-9,12-Octadecadieonate; Friedoolean-8-en-3-one; friedours-7-en-3-one; 13,27-Cyclosuran-3-one; Stigmaste-7,25-dien-3-ol (3β, 5α); Stigmasta-7,16-dien-3-ol; chrondrillasterol in BH seeds and CC roots extracts respectively.

Conclusion: Eluted components from the extracts could provide further researchers to work with various pharmacological activities related models and studies.

Keywords: 25-dien-3-ol; Apocynaceae; Benincasa hispida; Carissa congesta; Cururbitaceae; Stigmaste-7; chrondrillasterol; gas chromatography; lupeol; mass spectroscopy.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Mass spectrum of Benincasa hispida extract
Figure 2
Figure 2
Mass spectrum showing presence of 2,4-di-tert-butylphenyl 5-hydroxypentanoate (2) in Benincasa hispida extract
Figure 3
Figure 3
Mass spectrum showing presence of Palmitic acid (3) in Benincasa hispida extract
Figure 4
Figure 4
Mass spectrum showing presence of 2-Ethylhexyl hepatdecyl sulfite (4) in Benincasa hispida extract
Figure 5
Figure 5
Mass spectrum showing presence of 6-Methyltridecane (5) in Benincasa hispida extract
Figure 6
Figure 6
Mass spectrum showing presence of Tridecane (6) in Benincasa hispida extract
Figure 7
Figure 7
Mass spectrum showing presence of 9,12-Octadeca-9,12-dienoyl chloride (7) in Benincasa hispida extract
Figure 8
Figure 8
Mass spectrum showing presence of Hexadecanoic acid, 3-(trimethylsilyl)oxy]propyl ester (8) in Benincasa hispida extract
Figure 9
Figure 9
Mass spectrum showing presence of 9,12-Octadecadenoic acid (Z,Z), 2-Hydroxy-1-(hyroxymethylethyl) ester (9) in Benincasa hispida extract
Figure 10
Figure 10
Mass spectrum showing presence of 9,12-Octadecadenoic acid (Z,Z), 2,3-dihydroxy propyl ester (10) in Benincasa hispida extract
Figure 11
Figure 11
Mass spectrum showing presence of n-propyl-9,12-octadecadienoate (11) in Benincasa hispida extract
Figure 12
Figure 12
Mass spectrum showing presence of lupeol (12) in Benincasa hispida extract
Figure 13
Figure 13
Mass spectrum showing presence of Taraxasterol (13) in Benincasa hispida extract
Figure 14
Figure 14
Mass spectrum showing presence of 6a,14a-Methanopicene (14) in Benincasa hispida extract
Figure 15
Figure 15
Mass spectrum of Carissa congesta extract
Figure 16
Figure 16
Mass spectrum showing presence of 9-Octadecene (15) in Carissa congesta extract
Figure 17
Figure 17
Mass spectrum showing presence of 2-Isopropenyl-5-methyl-6-hepten-1-ol (16) in Carissa congesta extract
Figure 18
Figure 18
Mass spectrum showing presence of 2-Hydroxy-1-(hydroxymethyl)ethyl ether (17) in Carissa congesta extract
Figure 19
Figure 19
Mass spectrum showing presence of Butyl 9,12-octadecadieonate (18) in Carissa congesta extract
Figure 20
Figure 20
Mass spectrum showing presence of Friedoolean-8-en-3-one (19) in Carissa congesta extract
Figure 21
Figure 21
Mass spectrum showing presence of Friedours-7-en-3-one (20) in Carissa congesta extract
Figure 22
Figure 22
Mass spectrum showing presence of 13,27-Cyclosuran-3-one (21) in Carissa congesta extract
Figure 23
Figure 23
Mass spectrum showing presence of (3 β,5α)-Stigma-7,25-dien-3-ol (22) in Carissa congesta extract
Figure 24
Figure 24
Mass spectrum showing presence of (3 β,5α)-Stigma-7,16-dien-3-ol (23) in Carissa congesta extract
Figure 25
Figure 25
Mass spectrum showing presence of chrondrillasterol (24) in Carissa congesta extract
Figure 26
Figure 26
Graphical Abstract
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