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. 2015 Apr 15;8(4):5272-8.
eCollection 2015.

Investigation of RNA interference suppression of matrix metalloproteinase-9 in mouse model of atherosclerosis

Zhe-Xiu Jin  1 , Qiang Xiong  1 , Fang Jia  1 , Chun-Ling Sun  1 , Hong-Tao Zhu  1 , Fu-Sheng Ke  1
Affiliations

Investigation of RNA interference suppression of matrix metalloproteinase-9 in mouse model of atherosclerosis

Zhe-Xiu Jin et al. Int J Clin Exp Med. .

Abstract

Objective: To investigate the effect of RNA interference of matrix metalloproteinase (MMP)-9 on atherosclerosis on atherosclerosis in apolipoprotein E (ApoE)-/- mouse.

Methods: ApoE-/- mouse strain and three cell lines (293T, NIH3T3 and Raw264.7) were used in the present study to investigate the effect of MMP-9 silencing by RNA interference. Thirty 10-week-old ApoE-/- mice were randomly assigned to a control group, lentiviruses with naked vector group and Lentiviruses-MMP-9 intervention group (n = 10). Aortic atherosclerotic plaques of the mice were stained with immunohistochemical techniques, the MMP-9 and high-sensitivity C-reactive protein levels of three groups were detected simultaneously. Expression of MMP-9 was significantly down-regulated in interference group. MMP-9 and high-sensitivity C-reactive protein levels in MMP-9 interference group were significantly lower than that of the control group.

Conclusion: The expression of MMP-9 is closely related to vulnerability of atherosclerotic plaques. Silencing of MMP-9 expression acts as a positive role in maintenance of atherosclerotic plaque stability. The present study provides novel experimental insight for the treatment of vulnerable plaques in atherosclerosis.

Keywords: Atherosclerosis; MMP-9; RNA interference; vulnerability.

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Figures

Figure 1
Figure 1
The RT-PCR results of MMP-9 mRNA gene in NIN3T3 cells and Raw264.7 cells. A: MMP-9 expression in NIN3T3 cells. B: MMP-9 expression in Raw264.7 cells. The expression of MMP-9 mRNA gene in KD group was significantly decreased in NIN3T3 cells and Raw264.7 cells. (CON: normal control group; NC: Negative control group; KD: RNAi group).
Figure 2
Figure 2
Western blotting results of detecting MMp-9 knock down level. PC: MMP-9 positive control; NC1: negative control group; NC2: negative control group with effective interference vector for mouse GAPDH; 1#: Treated with 1# RNAi vector; 2#: Treated with 2# RNAi vector; 3#: Treated with 3# RNAi vector. 2# RNAi vector has a significant knock down effect on MMP-9.
See this image and copyright information in PMC

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