Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Jul 1;10(7):e0126370.
doi: 10.1371/journal.pone.0126370. eCollection 2015.

Salivary Matrix Metalloproteinase-8 and -9 and Myeloperoxidase in Relation to Coronary Heart and Periodontal Diseases: A Subgroup Report from the PAROKRANK Study (Periodontitis and Its Relation to Coronary Artery Disease)

Nilminie Rathnayake  1 , Anders Gustafsson  1 , Anna Norhammar  2 , Barbro Kjellström  2 , Björn Klinge  3 , Lars Rydén  2 , Taina Tervahartiala  4 , Timo Sorsa  5 ; PAROKRANK Steering Group
Affiliations

Salivary Matrix Metalloproteinase-8 and -9 and Myeloperoxidase in Relation to Coronary Heart and Periodontal Diseases: A Subgroup Report from the PAROKRANK Study (Periodontitis and Its Relation to Coronary Artery Disease)

Nilminie Rathnayake et al. PLoS One. .

Abstract

Background and objective: Matrix metalloproteinase (MMP) -8, -9 and myeloperoxidase (MPO) are inflammatory mediators. The potential associations between MMP-8, -9, MPO and their abilities to reflect cardiovascular risk remains to be evaluated in saliva. The objective of this study was to investigate the levels and associations of salivary MMP-8, -9, MPO and tissue inhibitors of metalloproteinase (TIMP)-1 in myocardial infarction (MI) patients and controls with or without periodontitis.

Materials and methods: 200 patients with a first MI admitted to coronary care units in Sweden from May 2010 to December 2011 and 200 controls matched for age, gender, residential area and without previous MI were included. Dental examination and saliva sample collection was performed 6-10 weeks after the MI in patients and at baseline in controls. The biomarkers MMP -8, -9, MPO and TIMP-1 were analyzed by time-resolved immunofluorescence assay (IFMA), Western blot and Enzyme-Linked ImmunoSorbent Assay (ELISA).

Results: After compensation for gingivitis, gingival pockets and smoking, the mean salivary levels of MMP-8 (543 vs 440 ng/mL, p = 0.003) and MPO (1899 vs 1637 ng/mL, p = 0.02) were higher in non-MI subjects compared to MI patients. MMP-8, -9 and MPO correlated positively with clinical signs of gingival/periodontal inflammation while TIMP-1 correlated mainly negatively with these signs. The levels of latent and active forms of MMP-8 did not differ between the MI and non-MI groups. Additionally, MMP-8, MPO levels and MMP-8/TIMP-1 ratio were significantly higher in men compared to women with MI.

Conclusions: This study shows that salivary levels of the analyzed biomarkers are associated with periodontal status. However, these biomarkers could not differentiate between patients with or without a MI. These findings illustrate the importance to consider the influence of oral conditions when analyzing levels of inflammatory salivary biomarkers.

PubMed Disclaimer

Conflict of interest statement

Competing Interests: The authors have read the journal's policy and the authors of this manuscript have the following competing interests: Prof. Timo Sorsa, University of Helsinki, Finland, holds US-Patents 5736341, 5652227, 5866432 and 6143476 on/describing/addressing technology on oral fluid diagnostic MMP-8 immunoassay, and this technology has been utilized in the authors’ paper. The patent is owned by Oy Medixbiochemica Ab/Ltd, Kauniainen, Finland, and according to the contract between Prof. Timo Sorsa and Medix Biochemica Oy/Ab/Ltd Prof. Timo Sorsa has received royalties from the Medix-company. Prof. Timo Sorsa confirms that this does not alter adherence to all PLOS ONE polices on sharing data and materials.

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Richards D (2014) Review finds that severe periodontitis affects 11% of the world population. Evid Based Dent 15:70–71. 10.1038/sj.ebd.6401037 - DOI - PubMed
    1. Hugoson A, Norderyd O (2008) Has the prevalence of periodontitis changed during the last 30 years? J Clin Periodontol 35:338–345. 10.1111/j.1600-051X.2008.01279.x - DOI - PubMed
    1. Bahekar AA, Singh S, Saha S, Molnar J, Arora R (2007) The prevalence and incidence of coronary heart disease is significantly increased in periodontitis: a meta-analysis. Am Heart J 154:830–837. - PubMed
    1. Uitto VJ, Overvall Cm, McCulloch C (2003) Proteolytic host enzymes in gingival crevicular fluid. Periodontol 2000 31:77–104. - PubMed
    1. Amalinei C, Caruntu ID, Ginsca SE, Balan RA (2010) Matrix metalloproteinase involvement in pathological condition. Rom J Morphol Embryol 52:215–228. - PubMed

Publication types

MeSH terms