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Comment
. 2015 Jul 7;112(27):8166-7.
doi: 10.1073/pnas.1510127112. Epub 2015 Jul 1.

Pre-T-cell receptor binds MHC: Implications for thymocyte signaling and selection

Xinbo Yang  1 , Roy A Mariuzza  2
Affiliations
Comment

Pre-T-cell receptor binds MHC: Implications for thymocyte signaling and selection

Xinbo Yang et al. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1.
Fig. 1.
PreTCR structure and interactions. (A) Crystal structure of the preTCR (PDB ID code 3OF6) (1). Regions of Vβ found to interact with pMHC in NMR titration experiments (CDR1, CDR3, and Vβ patch) (2) are depicted as molecular surfaces. (B) Cartoon representation of the preTCR structure in A showing the proposed interaction with a pMHC class I (pMHC I) self-ligand. The MHC class I heavy chain is drawn as three light blue ovals; β2-microglobulin (β2m) is an orange sphere; MHC-bound peptide is a red ball. The preTCR is perpendicular to the plane of the thymocyte membrane. Associated with the Cβ domain at the base of the preTCR is the CD3εγ heterodimer (9, 10). For clarity, other CD3 subunits (CD3εδ and CD3ζζ) are not shown. (C) Head-to-tail preTCR crystallographic dimer (1). The dimer lies parallel to the membrane with Vβ CDRs inaccessible to pMHC. This arrangement cannot accommodate CD3εγ in the manner shown in B. (D) Side-by-side crystallographic dimer. In this case, two preTCR stand upright on the membrane. The Vβ CDRs are available to bind pMHC and CD3εγ can associate with Cβ as in B.
See this image and copyright information in PMC

Comment on

References

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    1. Yamasaki S, et al. Mechanistic basis of pre-T cell receptor-mediated autonomous signaling critical for thymocyte development. Nat Immunol. 2006;7(1):67–75. - PubMed

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