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Comparative Study
. 2015 Sep;53(9):3032-4.
doi: 10.1128/JCM.01691-15. Epub 2015 Jul 1.

Comparison of Three Commercial Molecular Assays for Detection of Rifampin and Isoniazid Resistance among Mycobacterium tuberculosis Isolates in a High-HIV-Prevalence Setting

K Strydom  1 , F Ismail  2 , M M Z Matabane  2 , O Onwuegbuna  3 , S V Omar  4 , N Ismail  5
Affiliations
Comparative Study

Comparison of Three Commercial Molecular Assays for Detection of Rifampin and Isoniazid Resistance among Mycobacterium tuberculosis Isolates in a High-HIV-Prevalence Setting

K Strydom et al. J Clin Microbiol. 2015 Sep.

Abstract

In a head-to-head comparison of the MTBDRplus version 2.0 (Hain Lifescience), the Xpert MTB/RIF (Cepheid), and the Anyplex MTB/NTM (Seegene) assays, we demonstrated equal sensitivity (59/61; 96.7%) and specificity (53/54; 98.1%) for detecting rifampin resistance with further analysis of discordances. The Xpert assay does not detect isoniazid resistance while the Anyplex assay showed high false positivity.

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References

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