Changes in FABP1 and gastrin receptor expression in the testes of rats that have undergone electrical injury

Abstract

Testicular trauma may occur due to accidental electrical injury. The aim of this study was to investigate alterations in the levels of fatty acid-binding protein 1 (FABP1) and gastrin receptor (gastrin R) in the testes following electrical injury. Sprague-Dawley rats were divided into control, fatal electrocution (220 V, 50 Hz, 60 sec) and electrical injury (220 V, 50 Hz, 60 sec) groups (n=8 per group). The animals in the fatal electrocution and electrical injury groups were deeply anesthetized with sodium pentobarbital prior to each treatment, in which the current was delivered via an anode connected to the left foreleg and a cathode to the right hindleg. The rats that survived were subsequently sacrificed by cervical dislocation. Control animals received cervical dislocation alone. Immunohistochemical analysis was performed to evaluate the protein expression of FABP1 and gastrin R in the testes. Sections were evaluated by digital image analysis. The expression levels of FABP1 and gastrin R were significantly increased following electrical injury, supported by an increase in the integrated optical density (IOD) when compared with that in the control group (P<0.05). However, no significant difference was found in FABP1 and gastrin R expression levels between the fatal electrocution and control groups. In summary, the protein expression levels of FABP1 and gastrin R were found to be significantly altered by electrical injury, suggesting that these two proteins may be important in underlying mechanisms of testicular injury during electrical injury. The findings indicate that such alterations would be reflected in abnormal testicular function.

Keywords: electrical injury; fatty acid-binding protein 1; gastrin receptor; rats; testis.

Figure 1.

Effect of electrical injury on FABP1 expression. Photomicrographs display a representative distribution of positive FABP1 expression in the testes of (A) control, (B) fatal electrocution and (C) electrical injury group rats. Magnification, ×200. Positive immunostaining appears as brown staining. Total FABP1 expression in rat testes subjected to electrical injury was significantly higher compared with that in control testes. FABP1, fatty acid-binding protein 1.

Figure 2.

Effect of electrical injury on gastrin receptor (gastrin R) expression. Photomicrographs showing a representative distribution of positive gastrin R expression in the testes of (A) control, (B) fatal electrocution and (C) electrical injury group rats. Magnification, ×200. Positive immunostaining appears as brown staining. Total gastrin R expression in rat testes subjected to electrical injury was significantly higher compared with that in control testes.