Nano-Synthetic Devices in Leishmaniasis: A Bioinformatics Approach

Abstract

Synthetic biology is an investigative and constructive means of understanding the complexities of biology. Substantial progress in the fields has resulted in the creation of synthetic gene circuits, which when uploaded into the appropriate nanoliposomal vehicle, can be used for a tunable response in a cell. These tunable elements can be applied to treat diseased condition for a transition to a healthy state. Though in its nascent stage of development synthetic biology is beginning to use its constructs to bring engineering approaches into biomedicine for treatment of infectious disease leishmaniasis.

Keywords: Leishmania; bioinformatics; nanodevices; nanotechnology; synthetic biology.

Figure 1

Programmable synthetic constructs can be used to assemble nanostructures within living cells in a controlled fashion for leishmanicidal effects; (A) Raw sequence data of proteins, DNA, and RNA can be retrieved from databases; (B) Raw sequence data analysis for selection of a good sequence for nanostructure design followed by designing, modeling, simulation, and visualization of bionanosystems; (C) Computational analysis of the nanostructures for stability, robustness, and evolvability; (D) Generating sequence for tailor made nano-synthetic constructs; (E) Selection of a suitable chassis microorganism for uploading the synthetic construct; (F) Optimum chassis uploaded with the construct for the synthesis of combinatorial nanostructures; (G) Application of the nanostructures in the form of vaccines, aptamers, immune modulators to leishmanial parasite for a leishmanicidal effect.