Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Oct;128(10):1945-56.
doi: 10.1007/s00122-015-2558-1. Epub 2015 Jul 8.

QTL mapping of thrips resistance in pepper

Awang Maharijaya  1   2 , Ben Vosman  1 , Greet Steenhuis-Broers  1 , Koen Pelgrom  1 , Agus Purwito  2 , Richard G F Visser  1 , Roeland E Voorrips  3
Affiliations

QTL mapping of thrips resistance in pepper

Awang Maharijaya et al. Theor Appl Genet. 2015 Oct.

Abstract

A QTL for thrips resistance on pepper chromosome 6 was identified and validated. This QTL affects thrips larval development and explains 50% of the variation. Thrips is one of the most damaging pests in pepper (Capsicum). Resistance to thrips was identified in Capsicum annuum. This study was aimed at the elucidation of the genetic background of thrips resistance in Capsicum through QTL mapping. The QTL analysis was carried out for Frankliniella occidentalis resistance in an F2 population consisting of 196 plants derived from an interspecific cross between the highly resistant C. annuum AC 1979 as female parent and the highly susceptible C. chinense 4661 as male parent. Fifty-seven SSR, 109 AFLP, and 5 SNP markers were used to construct a genetic map with a total length of 1636 cM. Damage caused by larvae and the survival of first and second instar larval stages observed in a no-choice test were used as parameters of resistance. Interval mapping detected one QTL for each of these parameters, all co-localizing near the same marker on chromosome 6. Use of this marker as co-factor in a multiple-QTL mapping analysis failed to uncover any additional QTLs. This QTL explained about 50% of the genetic variation, and the resistance allele of this QTL was inherited from the resistant parent. Thrips resistance was not linked to trichome density.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Frequency distributions for overall damage caused by first instar larva, survival to L2 (second larval stage), and survival to pre-pupa in F2 population from a cross between resistant and susceptible accessions of pepper. Arrows indicate the approximate means of the resistant parent (R), susceptible parent (S), F1 and F2 population
Fig. 2
Fig. 2
LOD profiles and 1-LOD and 2-LOD support intervals for resistance QTLs on chromosomes 5 and 6. Solid, dashed, and dot-dashed lines represent the profiles for damage, survival to L2, and survival to pre-pupae, respectively, after inoculation with newly emerged L1 larvae of F. occidentalis. The dotted line at LOD 3.6 represents the LOD threshold. On chromosome 5 no QTLs were detected for these traits
Fig. 3
Fig. 3
LOD profiles and 1-LOD and 2-LOD support intervals for QTL for trichome density on chromosome 10. Solid, dashes, and dot-dashed lines represent the trichome density at early vegetative, late vegetative, and reproductive stage, respectively. The dotted line at LOD 3.6 represents the LOD threshold
See this image and copyright information in PMC

References

    1. Allard RW. Principles of plant breeding. 2. New York: John Willey & Sons Inc.; 1999.
    1. Barchi L, Bonnet J, Boudet C, Signoret P, Nagy I, Lanteri S, Palloix A, Lefebvre V. A high-resolution, intraspecific linkage map of pepper (Capsicum annuum L.) and selection of reduced recombinant inbred line subsets for fast mapping. Genome. 2007;50:51–60. doi: 10.1139/g06-140. - DOI - PubMed
    1. Borovsky Y, Paran I. Characterization of fs10.1, a major QTL controlling fruit elongation in Capsicum. Theor Appl Genet. 2011;123:657–665. doi: 10.1007/s00122-011-1615-7. - DOI - PubMed
    1. Capinera JL. In: Order thysanoptera-thrips: Handbook of vegetable pests. Capinera JL, editor. San Diego: Academic Press; 2001. pp. 535–550.
    1. Fery RL, Schalk JM. Resistance in pepper (Capsicum annuum L.) to western flower thrips [Frankliniella occidentalis (Pergande)] HortScience. 1991;26:1073–1074.

Publication types

Substances