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Comment
. 2016 Apr;13(2):152-3.
doi: 10.1089/zeb.2015.29000.sha. Epub 2015 Jul 8.

Rapid Reverse Genetic Screening Using CRISPR in Zebrafish

Arish N Shah  1 , Crystal F Davey  1 , Alex C Whitebirch  1 , Adam C Miller  1 , Cecilia B Moens  1
Affiliations
Comment

Rapid Reverse Genetic Screening Using CRISPR in Zebrafish

Arish N Shah et al. Zebrafish. 2016 Apr.
No abstract available

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Figures

<b>FIG. 1.</b>
FIG. 1.
Pools of single guide RNAs (sgRNAs) designed to target multiple candidate genes, along with Cas9-encoding mRNA, are injected into the single-cell stage embryo. Mutagenesis (depicted as large X) occurs at each of the pool targets (depicted as boxes on chromosomes) and mutant phenotypes (depicted as a loss of synapses) can be scored in the injected (F0) animals. Any sgRNA pool that caused a phenotype can be split (demultiplexed) with each sgRNA being reinjected individually to determine the sgRNA/gene responsible for the phenotype. Color images available online at www.liebertpub.com/zeb
See this image and copyright information in PMC

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References

    1. Shah AN, et al. . Rapid reverse genetic screening using CRISPR in zebrafish. Nat Methods 2015;12:535–540 - PMC - PubMed

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