Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014;39(3):338-44.
doi: 10.5114/ceji.2014.45945. Epub 2014 Oct 14.

CD4+CD25(high), CD8+CD28- cells and thyroid autoantibodies in breast cancer patients

Oğuz Biylgi  1 , Bülent Karagöz  1 , Orhan Türken  2 , Mustafa Gültepe  3 , Alpaslan Özgün  1 , Tolga Tunçel  1 , Levent Emirzeoğlu  1 , Serkan Çelik  1 , Tuba Müftüoğlu  3 , Emin Gökhan Kandemir  4
Affiliations

CD4+CD25(high), CD8+CD28- cells and thyroid autoantibodies in breast cancer patients

Oğuz Biylgi et al. Cent Eur J Immunol. 2014.

Abstract

Aim of the study: To investigate the percentage of CD4+CD25(high) cells (including Treg cells) and CD8+CD28- cells in breast cancer patients with and without high levels of autoimmune thyroid antibodies.

Material and methods: Thirty-five women with breast cancer (9 of them having high thyroid antibodies) and fourteen healthy subjects were enrolled in this study. Flow cytometry was used to count CD4+CD25(high) cells and CD8+CD28- suppressive cells (CD8 cell subtypes).

Results: In the patient group, the percentage of CD28- cells in CD8+ lymphocytes were higher [67.50% (55.1180.33) vs. 51.56% (42.5766.38); p = 0.021] and the percentage of CD28+CD45RO- cells (memory cells) in CD8+ lymphocytes were lower than in the control group. CD4+CD25(high) cell percentage in CD4+ lymphocytes was elevated in the patient group [6.44% (4.528.74) vs. 2.97% (1.724.34); p < 0.001]. When the cytometric parameters were compared between patients (with high vs. normal thyroid antibodies), the distribution of CD8+ cell subgroups was also similar. CD4+CD25(high) cells among CD4+ lymphocytes were decreased in patients with high levels of thyroid antibodies [5.19% (3.426.17) vs. 6.99% (4.829.95); p = 0.043].

Conclusions: CD4+CD25(high) cells may play a role in autoimmunity of breast cancer patients, and may be a predictive marker. Advanced studies which evaluate the possible links between regulatory cells and autoimmunity should be established in cancer patients.

Keywords: Treg cells; autoimmunity; breast cancer; thyroid antibody.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
CD8+ lymphocyte subtypes in FACS. The lymphocytes (in FS/SS histogram) and CD8bright cells (in lymphocytes) were gated. The percentage of CD8+ lymphocyte subtypes (%) was calculated in CD8+ lymphocyte gated CD28/ CD45RO histogram
Fig. 2
Fig. 2
CD4+CD25+ cells in FACS. The lymphocytes (in FS/SS histogram) and CD4+ cells (in lymphocytes) were gated. The percentage of CD4+CD25high cells (%) was calculated in CD4+ lymphocyte gated CD4/CD25 histogram
Fig. 3
Fig. 3
CD4+CD25high cell percentages (%) in breast cancer patients with and without high thyroid autoantibodies and healthy women. Treg cells among CD4+ lymphocytes were decreased in patients with high levels of thyroid antibodies compared in patients without
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Turken O, NarIn Y, DemIrbas S, et al. Breast cancer in association with thyroid disorders. Breast Cancer Res. 2003;5:R110–R113. - PMC - PubMed
    1. Grani G, Dicorato P, Dainelli M, et al. Thyroid diseases in women with breast cancer. Clin Ter. 2012;163:e401–e404. - PubMed
    1. Hardefeldt PJ, Eslick GD, Edirimanne S. Benign thyroid disease is associated with breast cancer: a meta-analysis. Breast Cancer Res Treat. 2012;133:1169–1177. - PubMed
    1. Penhale WJ, Farmer A, Irvine WJ. Thyroiditis in T cell-depleted rats. Influence of strain, radiation dose, adjuvants and antilymphocyte serum. Clin Exp Immunol. 1975;21:362–375. - PMC - PubMed
    1. Morris GP, Kong YC. Tolerance to autoimmune thyroiditis: (CD4+)CD25+ regulatory T cells influence susceptibility but do not supersede MHC class II restriction. Front Biosci. 2006;11:1234–1243. - PubMed

LinkOut - more resources