Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Dec;24(12):1053-5.
doi: 10.1007/BF02544079.

A method for the simultaneous analysis of unconjugated and glycine-conjugated bile acids by capillary gas-liquid chromatography

T Iida  1 , T Itoh, K Hagiwara, F C Chang, J Goto, T Nambara
Affiliations

A method for the simultaneous analysis of unconjugated and glycine-conjugated bile acids by capillary gas-liquid chromatography

T Iida et al. Lipids. 1989 Dec.

Abstract

A method for the simultaneous analysis of unconjugated and glycine-conjugated bile acids by means of capillary gas-liquid chromatography without need for prior deconjugation is described. The method involves: i) the use of an aluminum-clad fused-silica capillary column coated with a very thin film (0.1 micron) of a highly thermostable bonded and crosslinked methyl polysiloxane, and ii) the analysis of the bile acids as their methyl ester-dimethylethylsilyl ether derivatives. This method, used to separate the major free and glycine-conjugated bile acids from human gall bladder bile, should be applicable for the analysis of other biological fluids.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. J Lipid Res. 1977 May;18(3):404-7 - PubMed
    1. J Lipid Res. 1983 May;24(5):491-511 - PubMed
    1. J Lipid Res. 1978 Aug;19(6):771-4 - PubMed
    1. J Chromatogr. 1985 Oct 11;343(2):259-70 - PubMed
    1. Clin Chim Acta. 1978 Jul 1;87(1):141-7 - PubMed

Publication types

LinkOut - more resources