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. 2015 Jul 9;16(1):512.
doi: 10.1186/s12864-015-1735-6.

Genome-wide identification and Phylogenic analysis of kelch motif containing ACBP in Brassica napus

Nadia Haingotiana Raboanatahiry  1   2 , Yongtai Yin  3   4 , Li Chen  5   6 , Maoteng Li  7   8
Affiliations

Genome-wide identification and Phylogenic analysis of kelch motif containing ACBP in Brassica napus

Nadia Haingotiana Raboanatahiry et al. BMC Genomics. .

Abstract

Background: Acyl-coA binding proteins (ACBPs) bind long chain acyl-CoA esters with very high affinity. Their possible involvement in fatty acid transportation from the plastid to the endoplasmic reticulum, prior to the formation of triacylglycerol has been suggested. Four classes of ACBPs were identified in Arabidopsis thaliana: the small ACBPs, the large ACBPs, the ankyrin repeats containing ACBPs and the kelch motif containing ACBPs. They differed in structure and in size, and showed multiple important functions. In the present study, Brassica napus ACBPs were identified and characterized.

Results: Eight copies of kelch motif ACBPs were cloned, it showed that B. napus ACBPs shared high amino acid sequence identity with A. thaliana, Brassica rapa and Brassica oleracea. Furthermore, phylogeny based on domain structure and comparison map showed the relationship and the evolution of ACBPs within Brassicaceae family: ACBPs evolved into four separate classes with different structure. Chromosome locations comparison showed conserved syntenic blocks.

Conclusions: ACBPs were highly conserved in Brassicaceae. They evolved from a common ancestor, but domain duplication and rearrangement might separate them into four distinct classes, with different structure and functions. Otherwise, B. napus inherited kelch motif ACBPs from ancestor conserving chromosomal location, emphasizing preserved synteny block region. This study provided a first insight for exploring ACBPs in B. napus, which supplies a valuable tool for crop improvement in agriculture.

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Figures

Fig. 1
Fig. 1
Domain structure of AtACBPs and BnACBPs. The architecture was generated by using Batch CD-search from NCBI database. ACBD are labeled in yellow, ankyrin repeats domains are in green and kelch domains are in blue
Fig. 2
Fig. 2
Evolutionary relationships of land plant ACBPs. The evolutionary history was inferred by using the Maximum Likelihood method based on Equal input model. The analysis involved 64 amino acid sequences. Evolutionary analyses were conducted in MEGA6
Fig. 3
Fig. 3
Comparison maps of kelch motif AtACBP, BrACBP, BoACBP and BnACBP. Maps were built manually, associating the homologue genes from each species. ACBPs are in bold. B. napus chromosomes are in yellow: this figure represents AIS76194 and AIS76199
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