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. 2015 Jul;10(1):359-363.
doi: 10.3892/ol.2015.3240. Epub 2015 May 20.

Significance of gastrin-releasing peptide in ovarian cancer ES2 cells

Yanyan Jia  1 , Huirong Shi  1 , Dongmei Fan  2
Affiliations

Significance of gastrin-releasing peptide in ovarian cancer ES2 cells

Yanyan Jia et al. Oncol Lett. 2015 Jul.

Abstract

The present study aimed to investigate the effect of gastrin-releasing peptide (GRP) on the proliferation and invasion of ovarian cancer ES2 cells. The ovarian cancer ES2 cells were transfected with small interfering RNA against GRP. Cell proliferation was assessed using the Trypan blue assay, apoptosis was determined using propidium iodide/fluorescein isothiocyanate and flow cytometry, and the invasion ability was detected using the Transwell assay. The results revealed that the expression of GRP significantly decreased following transfection with GRP-short hairpin RNA. Furthermore, the silencing of GRP resulted in increased apoptosis and a reduced invasive ability of the ES2 cells. It was concluded that GRP may regulate the proliferation and migration of human ovarian cancer cells, which indicates that GRP may be a potential novel target for the treatment of ovarian cancer.

Keywords: cell proliferation; gastrin-releasing peptide; ovarian cancer; small interfering RNA.

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Figures

Figure 1.
Figure 1.
Digestion of pGenesil-GRP-shRNA by SacI. Lanes 1 and 2, pGenesil-GRP-shRNA; lane 3, negative control; M, DNA marker with 2,000, 1,000, 750, 500, 250 and 100-bp bands (top to bottom). GRP, gastrin-releasing peptide; shRNA, short hairpin RNA.
Figure 2.
Figure 2.
Green fluorescent protein in ES2 cells transfected with pGenesil-GRP-shRNA. GRP, gastrin-releasing peptide; shRNA, short hairpin RNA.
Figure 3.
Figure 3.
Expression of GRP mRNA following silencing. Lane Ma,100bp Plus II DNA marker with 1,500, 1,000, 900, 800, 700, 500, 400, 300, 200 and 100-bp bands (top to bottom) Lane 1, untransfected group; lane 2, empty vector-transfected group; lane 3, GRP-shRNA-transfected group. GRP, gastrin-releasing peptide; shRNA, short hairpin RNA.
Figure 4.
Figure 4.
Protein levels of GRP following silencing. Lane 1, untransfected group; lane 2, empty vector-transfected group; lane 3, GRP-shRNA-transfected group. GRP, gastrin-releasing peptide; shRNA, short hairpin RNA.
Figure 5.
Figure 5.
GRP silencing-induced cell proliferation inhibition. GRP, gastrin-releasing peptide; shRNA, short hairpin RNA.
Figure 6.
Figure 6.
GRP silencing-induced apoptosis. (A) GRP-shRNA-transfected group, (B) vector-transfected group and (C) untransfected group. GRP, gastrin-releasing peptide; shRNA, short hairpin RNA; FITC, fluorescein isothiocyanate; PI, propidium iodide; PE, phycoerythrin.
Figure 7.
Figure 7.
Invasion ability of ES2 cells following GRP silencing. (A) Untransfected group, (B) vector-transfected group and (C) GRP-shRNA-transfected group. GRP, gastrin-releasing peptide; shRNA, short hairpin RNA.
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