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. 2015 Jul-Aug;16(4):791-7.
doi: 10.3348/kjr.2015.16.4.791. Epub 2015 Jul 1.

Permeability Parameters Measured with Dynamic Contrast-Enhanced MRI: Correlation with the Extravasation of Evans Blue in a Rat Model of Transient Cerebral Ischemia

Hyun Seok Choi  1 , Sung Soo Ahn  2 , Na-Young Shin  2 , Jinna Kim  2 , Jae Hyung Kim  3 , Jong Eun Lee  4 , Hye Yeon Lee  1 , Ji Hoe Heo  5 , Seung-Koo Lee  2
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Permeability Parameters Measured with Dynamic Contrast-Enhanced MRI: Correlation with the Extravasation of Evans Blue in a Rat Model of Transient Cerebral Ischemia

Hyun Seok Choi et al. Korean J Radiol. 2015 Jul-Aug.

Abstract

Objective: The purpose of this study was to correlate permeability parameters measured with dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) using a clinical 3-tesla scanner with extravasation of Evans blue in a rat model with transient cerebral ischemia.

Materials and methods: Sprague-Dawley rats (n = 13) with transient middle cerebral artery occlusion were imaged using a 3-tesla MRI with an 8-channel wrist coil. DCE-MRI was performed 12 hours, 18 hours, and 36 hours after reperfusion. Permeability parameters (K(trans), ve, and vp) from DCE-MRI were calculated. Evans blue was injected after DCE-MRI and extravasation of Evans blue was correlated as a reference with the integrity of the blood-brain barrier. Correlation analysis was performed between permeability parameters and the extravasation of Evans blue.

Results: All permeability parameters (K(trans), ve, and vp) showed a linear correlation with extravasation of Evans blue. Among them, K(trans) showed highest values of both the correlation coefficient and the coefficient of determination (0.687 and 0.473 respectively, p < 0.001).

Conclusion: Permeability parameters obtained by DCE-MRI at 3-T are well-correlated with Evans blue extravasation, and K(trans) shows the strongest correlation among the tested parameters.

Keywords: Animal models; Blood-brain barrier; Brain ischemia; Dynamic contrast-enhanced MRI; Kinetic modeling.

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Figures

Fig. 1
Fig. 1. Selection of arterial input function (AIF) for analysis of permeability parameters.
A. Region of interest was set at region of left cervical internal carotid artery (green color). Venous flow is depicted in blue. B. Time-concentration of gadolinium curve showed rapid upslope and early peak of gadolinium concentration (GD), suggestive of appropriate selection of arterial flow.
Fig. 2
Fig. 2. Analysis of optical density.
A. Posterior surface of sliced specimen (Bregma-1.60) was prepared for analysis of extravasation of Evans blue. ROIs were placed in right cortex (B) and basal ganglia (C) for measurement of optical density.
Fig. 3
Fig. 3. Extravasation of Evans blue and Ktrans map.
Extravasation of Evans blue was displayed on left column and Ktrans map on right column in 12 hours after reperfusion (A), 18 hours after reperfusion (B), and 36 hours after reperfusion (C).
Fig. 4
Fig. 4. Scatter plots and fitted linear correlation lines of optical density versus permeability parameters. Ktrans (A), ve (B), and vp (C).
See this image and copyright information in PMC

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