Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Jun 23;12(7):544-51.
doi: 10.7150/ijms.12217. eCollection 2015.

Cytological Kinetics of Periodontal Ligament in an Experimental Occlusal Trauma Model

Tatsuo Takaya  1 , Hiroaki Mimura  1 , Saeka Matsuda  2 , Keisuke Nakano  2 , Hidetsugu Tsujigiwa  3 , Mihoko Tomida  4 , Norimasa Okafuji  2 , Takeo Fujii  1 , Toshiyuki Kawakami  2
Affiliations

Cytological Kinetics of Periodontal Ligament in an Experimental Occlusal Trauma Model

Tatsuo Takaya et al. Int J Med Sci. .

Abstract

Using a model of experimental occlusal trauma in mice, we investigated cytological kinetics of periodontal ligament by means of histopathological, immunohistochemical, and photographical analysis methods. Periodontal ligament cells at furcation areas of molar teeth in the experimental group on day 4 showed a proliferation tendency of periodontal ligament cells. The cells with a round-shaped nucleus deeply stained the hematoxylin and increased within the day 4 specimens. Ki67 positive nuclei showed a prominent increase in the group on days 4 and 7. Green Fluorescent Protein (GFP) positivity also revealed cell movement but was slightly slow compared to Ki67. It indicated that restoration of mechanism seemed conspicuous by osteoclasts and macrophages from bone-marrow-derived cells for the periodontal ligament at the furcation area. It was suggested that the remodeling of periodontal ligament with cell acceleration was evoked from the experiment for the group on day 4 and after day 7. Periodontal ligament at the furcation area of the molar teeth in this experimental model recovered using the cells in situ and the bone-marrow-derived cells.

Keywords: Green fluorescent protein (GFP); Ki67; Mouse; Occlusal trauma; Periodontal tissue.

PubMed Disclaimer

Conflict of interest statement

Competing Interests: The authors have declared that no competing interest exists.

Figures

Figure 1
Figure 1
Experimental schema
Figure 2
Figure 2
Histopathological photograph of the observation site
Figure 3
Figure 3
Histopathology of control group specimen (A), experimental 4 day group specimen (B), experimental 7 day group specimen (C) and experimental 14 day group specimen (D). Scale bar: 50 µm.
Figure 4
Figure 4
Hemotoxylin-deeply-stained-portion sharing ratio
Figure 5
Figure 5
Ki67 IHC staining specimens. Control (A), Experimental 4 day (B), Experimental 7 day (C) and Experimental 14 day (D). Scale bar: 50µm.
Figure 6
Figure 6
Ki67-positive cell ratio
Figure 7
Figure 7
IHC staining of GFP. Control specimen (A), Experimental 4 day specimen (B), Experimental 7 day specimen (C) and Experimental 14 day specimen (D). Scale bar: 50 µm.
Figure 8
Figure 8
GFP positive area share ratio
See this image and copyright information in PMC

References

    1. Svanberg G. Influence of trauma from occlusion on the periodontium of dogs with normal or inflamed gingivae. Odontol Revy. 1974;25:165–178. - PubMed
    1. Stahl SS. Accommodation of the periodontium to occlusal trauma and inflammatory periodontal disease. Dent Clin North Am. 1975;19:531–542. - PubMed
    1. Lindhe J, Ericsson I. The influence of trauma from occlusion on reduced but healthy periodontal tissues in dogs. J Clin Periodontol. 1976;3:110–122. - PubMed
    1. Biancu S, Ericsson I, Lindhe J. Periodontal ligament tissue reactions to trauma and gingival inflammation. An experimental study in the beagle dog. J Clin Periodontol. 1995;22:772–779. - PubMed
    1. Glickman I, Smulow JB. Effect of excessive occlusal forces upon the pathway of gingival inflammation in humans. J Periodontol. 1965;36:141–147. - PubMed

Publication types

LinkOut - more resources