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. 1978;19(1):93-101.
doi: 10.1186/BF03547645.

The effect of pH and potassium phosphate buffer on the toxicity of cadmium for bacteria

H Korkeala, T J Pekkanen

The effect of pH and potassium phosphate buffer on the toxicity of cadmium for bacteria

H Korkeala et al. Acta Vet Scand. 1978.

Abstract
in English, German

An increase in the pH of Plate Count Agar led to increased toxicity of cadmium (Cd) for Micrococcus luteus ATCC 9341, Staphylococcus aureus ATCC 12600, Clostridium perfringens ATCC 8798, Escherichia coli ATCC 11775 and Pseudomonas aeruginosa ATCC 10145. The effect of the pH on toxicity was not clearly observed with Streptococcus bovis ATCC 9809 and was absent with Bacillus subtilis ATCC 8473.

When potassium phosphate buffer was added to the growth medium, the Cd toxicity for M. luteus and B. subtilis was enhanced. The toxicity of Cd for S. bovis decreased when potassium phosphate buffer was added to the medium. When the pH increased at differing phosphate concentrations, the sensitivity of M. luteus to Cd decreased.

In the authors’ opinion, the effect of pH on Cd toxicity for bacteria is due to a continuously increasing negative charge towards an alkaline value by most bacteria which increases the affinity of cations towards the cell wall.

Toxisiteten av kadmium (Gd) för Micrococcus luteus ATGG 9341, Staphylococcus aureus ATCG 12600, Clostridium perfringens ATCG 8798, Escherichia coli ATGG 11775 och Pseudomonas aeruginosa ATCG 10145 ökade vid stigande pH i bakterieräkningsmediet. Inverkan av pH på toxisiteten var mindre utpräglad för Streptococcus bovis ATCC 9809 och utblev för Bacillus subtilis ATCG 8473. När kaliumfosfatbuffer tillsattes i odlingsmediet tilltog toxisiteten av Gd för M. luteus och B. subtilis men minskade för S. bovis. När pH-värdet i mediet steg vid olika fosfatkonsentrationer minskade känsligheten av M. luteus för Gd. Författarna är av den åsikten att effekten av pH på toxisiteten av Gd är för de flesta mikrober förorsakad av den ökade negativa laddningen vid tilltagande alkalisitet, vilket ökar cellväggens affinitet för kationer.

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