Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Apr;25(2):e381.
doi: 10.5812/ijp.381. Epub 2015 Apr 18.

Serum Hsp70 Antigen: Early Diagnosis Marker in Perinatal Asphyxia

Hassan Boskabadi  1 , Masoud Omidian  1 , Shima Tavallai  2 , Shabnam Mohammadi  3 , Mostafa Parizadeh  2 , Majid Ghayour Mobarhan  2 , Gordon Aa Ferns  4
Affiliations

Serum Hsp70 Antigen: Early Diagnosis Marker in Perinatal Asphyxia

Hassan Boskabadi et al. Iran J Pediatr. 2015 Apr.

Abstract

Background: Perinatal asphyxia is an important cause of mortality and permanent neurological and developmental deficit. Early and accurate diagnosis would help to establish the likely prognosis and may also help in determining the most appropriate treatment. Studies in experimental animal models suggest that a protein called Hsp70 may be a good and potentially useful marker of cellular stress that may be clinically useful in determining the presence of neonatal asphyxia.

Objectives: Regarding the importance of early and accurate diagnosis of asphyxia, we conducted this study, which is the first investigation of the comparison of the serum Hsp70 antigen level between asphyxiated and healthy infants.

Patients and methods: In this observational study, the serum concentrations of Hsp70 antigen were compared between neonates suffering from perinatal asphyxia (n = 50) and normal neonates (n = 51). The inclusion criteria for the cases were neonates who had reached term and had at least two clinical criteria of asphyxia. Exclusion criteria were babies with gestational age < 37 weeks, infants with congenital abnormalities or positive blood culture. Exclusion criteria in this group were the requirement to hospital stay during first week of the life or babies whose mothers had difficulties during pregnancy or delivery. Term neonates without major anomalies who had asphyxia during delivery were enrolled in the first six hours after delivery, and control group consisted of healthy term neonates without problems and normal delivery process in the first week of life. The cord blood was taken during labor to measure Hsp70 antigen level by using an in-house ELISA (The enzyme-linked immunosorbent assay).

Results: The median values of serum anti Hsp70 titers were significantly higher in asphyxiated neonates compared with non-asphyxiated neonates (0.36 [0.04 - 1.14] vs 0.24 [0.01 - 0.63]). At cutoff point = 0.3125 ng/mL, sensitivity was 58% and specificity 76% based on ROC curve.

Conclusions: A significant difference between the serum concentrations of Hsp70 of the control and patient group was observed in this study. It is inferred serum concentrations of Hsp70 antigen may be a useful marker for the early diagnosis of that prenatal hypoxia.

Keywords: Asphyxia Neonatorum; HSP70 Heat-Shock Proteins; Hypoxia-Ischemia, Brain.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.. Receiver Operating Characteristics (ROC) Graph to Discriminate the Sensitivity and Specificity of Serum Hsp70 Level for the Diagnosis of Perinatal Asphyxia
See this image and copyright information in PMC

References

    1. Jojart G, Nagy G, Pasztor J, Kovacs Z. [Bilateral neonatal adrenal hemorrhage associated with hypoadrenalism]. Orv Hetil. 1992;133(19):1179–81. - PubMed
    1. Pierrat V, Haouari N, Liska A, Thomas D, Subtil D, Truffert P, Groupe d'Etudes en Epidemiologie P, et al. Prevalence, causes, and outcome at 2 years of age of newborn encephalopathy: population based study. Arch Dis Child Fetal Neonatal Ed. 2005;90(3):F257–61. doi: 10.1136/adc.2003.047985. - DOI - PMC - PubMed
    1. Black RE, Cousens S, Johnson HL, Lawn JE, Rudan I, Bassani DG, Jha P, Campbell H, Walker CF, Cibulskis R, Eisele T, Liu L, Mathers C, Child Health Epidemiology Reference Group of WHO, Unicef. et al. Global, regional, and national causes of child mortality in 2008: a systematic analysis. Lancet. 2010;375(9730):1969–87. doi: 10.1016/S0140-6736(10)60549-1. - DOI - PubMed
    1. Hagmann CF, Brotschi B, Bernet V, Latal B, Berger TM, Robertson NJ. Hypothermia for perinatal asphyxial encephalopathy. Swiss Med Wkly. 2011;141:w13145. doi: 10.4414/smw.2011.13145. - DOI - PubMed
    1. Million Death Study C, Bassani DG, Kumar R, Awasthi S, Morris SK, Paul VK, Shet A, Ram U, Gaffey MF, Black RE, Jha P, et al. Causes of neonatal and child mortality in India: a nationally representative mortality survey. Lancet. 2010;376(9755):1853–60. doi: 10.1016/S0140-6736(10)61461-4. - DOI - PMC - PubMed

LinkOut - more resources