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. 2016 Jan;54(1):22-7.
doi: 10.1093/chromsci/bmv100. Epub 2015 Jul 21.

Validation of a Stability-Indicating Method for Methylseleno-L-Cysteine (L-SeMC)

Kristin Canady  1 , Johnathan Cobb  1 , Peter Deardorff  1 , Jami Larson  2 , Jonathan M White  1 , Dan Boring  3
Affiliations

Validation of a Stability-Indicating Method for Methylseleno-L-Cysteine (L-SeMC)

Kristin Canady et al. J Chromatogr Sci. 2016 Jan.

Abstract

Methylseleno-L-cysteine (L-SeMC) is a naturally occurring amino acid analogue used as a general dietary supplement and is being explored as a chemopreventive agent. As a known dietary supplement, L-SeMC is not regulated as a pharmaceutical and there is a paucity of analytical methods available. To address the lack of methodology, a stability-indicating method was developed and validated to evaluate L-SeMC as both the bulk drug and formulated drug product (400 µg Se/capsule). The analytical approach presented is a simple, nonderivatization method that utilizes HPLC with ultraviolet detection at 220 nm. A C18 column with a volatile ion-pair agent and methanol mobile phase was used for the separation. The method accuracy was 99-100% from 0.05 to 0.15 mg/mL L-SeMC for the bulk drug, and 98-99% from 0.075 to 0.15 mg/mL L-SeMC for the drug product. Method precision was <1% for the bulk drug and was 3% for the drug product. The LOQ was 0.1 µg/mL L-SeMC or 0.002 µg L-SeMC on column.

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Figures

Figure 1.
Figure 1.
Chromatograms of l-SeMC reference standard (0.1 mg/mL) and diluent.
Figure 2.
Figure 2.
Forced degradation chromatograms.
See this image and copyright information in PMC

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