Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Dec;14(Suppl 1):24-30.
doi: 10.1007/s13191-014-0354-2. Epub 2014 Feb 7.

Effectiveness of Chemical and Microwave Disinfection on Denture Biofilm Fungi and the Influence of Disinfection on Denture Base Adaptation

Mohannad H Al-Saadi  1
Affiliations

Effectiveness of Chemical and Microwave Disinfection on Denture Biofilm Fungi and the Influence of Disinfection on Denture Base Adaptation

Mohannad H Al-Saadi. J Indian Prosthodont Soc. 2014 Dec.

Abstract

The aims of this study were to evaluate the effectiveness of six disinfection methods and the influence of these methods on the adaptation of maxillary dentures. Acrylic resin specimens contaminated with fungi were exposed to the following disinfection treatments: 1. microwave oven (900 W) at full power for 5 min (with soaking the specimen in 250 ml water), 2. microwave oven at medium power for 5 min (with soaking the specimen in 250 ml water), 3. sodium hypochlorite 5.25 % for 5 min, 4. diluted sodium hypochlorite 1:420 for 5 h, 5. Chlorhexidine gluconate for 5 h, 6. effervescent tablets for 15 min, 7. soaking in 250 ml tap water for 15 min. Colony forming units (CFUs) of remaining cells were counted and compared with t test (p ≤ 0.05). Dimensional stability was evaluated using aluminum die simulating the maxillary edentulous arch. Posterior palatal gaps were measured. Data were analyzed using one-way ANOVA test and t test (p ≤ 0.05). Microwave irradiation (at full or medium power) and sodium hypochlorite 5.25 % for 5 min were able to reduce the CFUs of fungi by more than 4 log10 whereas diluted sodium hypochlorite, chlorhexidine gluconate, and effervescent tablets did not achieve a reduction of >2.8, 2.68 and 1.66, respectively. For dimensional stability test, t test revealed significant difference between control group and the microwave at full power group (p = 0.000). Within the limits of this study, microwave oven at medium power and sodium hypochlorite (5.25 %) are effective and safe methods of disinfecting removable dentures.

Keywords: Base adaptation; Chemical disinfection; Dimensional stability; Fungi; Microwave; Sodium hypochlorite.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Colony forming units on Sabouraud agar plates were counted for every disinfection method
Fig. 2
Fig. 2
Aluminum die simulating the maxillary edentulous arch
Fig. 3
Fig. 3
Light microscope connected to computer used for measuring the posterior gaps
Fig. 4
Fig. 4
Microscopic examination showing Candida species
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Jeganathan S, Lin C. Denture stomatitis: a review of the aetiology, diagnosis and management. Aust Dent J. 1992;37:107–114. doi: 10.1111/j.1834-7819.1992.tb03046.x. - DOI - PubMed
    1. McCourtie J, Douglas L. Extracellular polymer of Candida albicans: isolation, analysis and role in adhesion. J Gen Microbiol. 1985;131:495–503. - PubMed
    1. Theilade E, Budtz-Jorgensen E. Predominant cultivable microflora of plaque on removable dentures in patients with denture induced stomatitis. Oral Microbiol Immunol. 1988;3:8–13. doi: 10.1111/j.1399-302X.1988.tb00597.x. - DOI - PubMed
    1. Kulak Y, Arikan A, Kazazoglu E. Existence of Candida albicans and microorganisms in denture stomatitis patients. J Oral Rehabil. 1997;24:788–790. doi: 10.1046/j.1365-2842.1997.00550.x. - DOI - PubMed
    1. Machado A, Breeding L, Vergani C, da Cruz Perez L. Hardness and surface roughness of reline and denture base acrylic resins after repeated disinfection procedures. J Prosthet Dent. 2009;102:115–122. doi: 10.1016/S0022-3913(09)60120-7. - DOI - PubMed

LinkOut - more resources