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Case Reports
. 2015 Aug;28(4):207-9.

Characterization of daptomycin non-susceptible Enterococcus faecium producing urinary tract infection in a renal transplant recipient

Antonio Sorlózano, Diana Panesso, José María Navarro-Marí, Cesar A Arias, José Gutiérrez-Fernández  1
Affiliations
Case Reports

Characterization of daptomycin non-susceptible Enterococcus faecium producing urinary tract infection in a renal transplant recipient

Antonio Sorlózano et al. Rev Esp Quimioter. 2015 Aug.

Abstract

Objectives: Characterization of a urine isolate of daptomycin non-susceptible Enterococcus faecium recovered from a patient with kidney transplantation and no history of daptomycin exposure.

Methods: After isolation in a urine sample, identification of E. faecium was confirmed by amplification of the E. faecium-specific gene encoding D-alanyl-D-alanine ligase (ddl) and daptomycin susceptibility testing was performed by E-test on cation-adjusted Mueller-Hinton agar. In order to determine the genetic bases of daptomycin resistance, the open reading frames of five genes previously associated with daptomycin resistance in enterococci were sequenced.

Results: Substitutions in the response regulator LiaR (S19F) and cardiolipin synthase (R218Q) were identified.

Conclusions: To the best of our knowledge, this is the first characterization of emerging daptomycin resistance in E. faecium in a Spanish hospital in the absence of daptomycin exposure and in a renal transplant recipient.

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References

    1. Canton R, Ruiz-Garbajosa P, Chaves RL, Johnson AP. A potential role for daptomycin in enterococcal infections: what is the evidence? J Antimicrob Chemother. 2010;65:1126–36. - PMC - PubMed
    1. Kelesidis T, Humphries R, Uslan DZ, Pegues DA. Daptomycin non-susceptible enterococci: an emerging challenge for clinicians. Clin Infect Dis. 2011;52:228–34. - PMC - PubMed
    1. Kelesidis T, Tewhey R, Humphries RM. Evolution of high-level daptomycin resistance in Enterococcus faecium during daptomycin therapy is associated with limited mutations in the bacterial genome. J Antimicrob Chemother. 2013;68:1926–8. - PMC - PubMed
    1. Munoz-Price LS, Lolans K, Quinn JP. Emergence of resistance to daptomycin during treatment of vancomycin-resistant Enterococcus faecalis infection. Clin Infect Dis. 2005;41:565–6. - PubMed
    1. Storm JC, Diekema DJ, Kroeger JS, Johnson SJ, Johannsson B. Daptomycin exposure precedes infection and/or colonization with daptomycin non-susceptible enterococcus. Antimicrob Resist Infect Control. 2012;1:19. - PMC - PubMed

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