Stem Cell Differentiation Stage Factors from Zebrafish Embryo: A Novel Strategy to Modulate the Fate of Normal and Pathological Human (Stem) Cells

Abstract

In spite of the growing body of evidence on the biology of the Zebrafish embryo and stem cells, including the use of Stem Cell Differentiation Stage Factors (SCDSFs) taken from Zebrafish embryo to impact cancer cell dynamics, comparatively little is known about the possibility to use these factors to modulate the homeostasis of normal human stem cells or to modulate the behavior of cells involved in different pathological conditions. In the present review we recall in a synthetic way the most important researches about the use of SCDSFs in reprogramming cancer cells and in modulating the high speed of multiplication of keratinocytes which is characteristic of some pathological diseases like psoriasis. Moreover we add here the results about the capability of SCDSFs in modulating the homeostasis of human adiposederived stem cells (hASCs) isolated from a fat tissue obtained with a novel-non enzymatic method and device. In addition we report the data not yet published about a first protein analysis of the SCDSFs and about their role in a pathological condition like neurodegeneration.

Fig. (1)

The effect of the mix ABC on CA1 area cell mortality after 1 hour of serum deprivation (*p=0.005).

Fig. (2)

The effect of the mix ABC on CA1 area cell mortality after 1 hour NMDA 50 μM treatment (*p=0.002; **p=0.01).

Fig. (3)

The effect of the mix ABC on CA1 area cell mortality after 1 hour NMDA 300 μM treatment (*p=0.0002; **p=0.009).

Fig. (4)

The effect of the single solutions A, B and C on CA1 area of hippocamp after 1 hour of serum deprivation. Values are expressed as percentage of samples treated with serum deprivation without SCDSFs.

Fig. (5)

The effect of the single solutions A, B and C on CA1 area of hippocamp after 1 hour of NMDA 50 μM treatment. Values are expressed as percentage of samples treated with NMDA 50 μM without SCDSFs.

Fig. (6)

Representative 12% SDS-PAGE gel of Zebrafish embryo extracts resuspended in a glycero-alcoholic solution. Lanes: M) Broad-range protein molecular weight markers (in kDa); 1) 50% epiboly stage proteins; 2) tail bud stage proteins; 3) 5 somites stage proteins; 4) 20 somites stage proteins; 5) pharingula stage proteins.