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. 2015 May 27:10:29.
doi: 10.1186/s40793-015-0010-4. eCollection 2015.

High quality draft genome sequence of Brachymonas chironomi AIMA4(T) (DSM 19884(T)) isolated from a Chironomus sp. egg mass

Sivan Laviad  1 , Alla Lapidus  2 , James Han  3 , Matthew Haynes  3 , Tbk Reddy  3 , Marcel Huntemann  3 , Amrita Pati  3 , Natalia N Ivanova  3 , Konstantinos Mavromatis  3 , Elke Lang  4 , Manfred Rohde  5 , Victor Markowitz  6 , Tanja Woyke  3 , Hans-Peter Klenk  4 , Nikos C Kyrpides  7 , Malka Halpern  8
Affiliations

High quality draft genome sequence of Brachymonas chironomi AIMA4(T) (DSM 19884(T)) isolated from a Chironomus sp. egg mass

Sivan Laviad et al. Stand Genomic Sci. .

Abstract

Brachymonas chironomi strain AIMA4(T) (Halpern et al., 2009) is a Gram-negative, non-motile, aerobic, chemoorganotroph bacterium. B. chironomi is a member of the Comamonadaceae, a family within the class Betaproteobacteria. This species was isolated from a chironomid (Diptera; Chironomidae) egg mass, sampled from a waste stabilization pond in northern Israel. Phylogenetic analysis based on the 16S rRNA gene sequences placed strain AIMA4(T) in the genus Brachymonas. Here we describe the features of this organism, together with the complete genome sequence and annotation. The DNA GC content is 63.5%. The chromosome length is 2,509,395 bp. It encodes 2,382 proteins and 68 RNA genes. Brachymonas chironomi genome is part of the Genomic Encyclopedia of Type Strains, Phase I: the one thousand microbial genomes (KMG) project.

Keywords: Brachymonas chironomi; Chironomid; Chironomus; Comamonadaceae; Egg mass; Toxicant.

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Figures

Figure 1
Figure 1
Phylogenetic tree highlighting the position of Brachymonas chironomi relative to the type strains of the other species within the family Comamonadaceae. The sequence alignments were performed by using the CLUSTAL W program and the tree was generated using the maximum likelihood method in MEGA 5 software. Bootstrap values (from 1,000 replicates) greater than 50% are shown at the branch points. The bar indicates a 1% sequence divergence.
Figure 2
Figure 2
Scanning electron micrograph of B.chironomi AIMA4T.
See this image and copyright information in PMC

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