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. 2015 Dec 2:214:1-3.
doi: 10.1016/j.ijfoodmicro.2015.07.016. Epub 2015 Jul 17.

Usefulness of pulsed-field gel electrophoresis profiles for the determination of Salmonella serovars

Chien-Shun Chiou  1 , Mia Torpdahl  2 , Ying-Shu Liao  3 , Chun-Hsing Liao  4 , Chi-Sen Tsao  5 , Shiu-Yun Liang  6 , You-Wun Wang  7 , Jung-Che Kuo  8 , Yen-Yi Liu  9
Affiliations

Usefulness of pulsed-field gel electrophoresis profiles for the determination of Salmonella serovars

Chien-Shun Chiou et al. Int J Food Microbiol. .

Abstract

We created a database consisting of a large number of Salmonella pulsed-field gel electrophoresis (PFGE) profiles covering a wide range of different serovars. This database was used for the prediction of the serovars based on the PFGE profiles for isolates from Taiwan and Denmark. The PFGE profiles proved very useful in the determination of a serovar although serovar prediction was more efficient for local isolates than those from a distant geographic area. To use a highly stringent band matching tolerance in the BioNumerics software is also important for the grouping of serovars.

Keywords: Molecular serotyping; Pulsed-field gel electrophoresis; Salmonella.

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