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. 2015;19(4):188-93.
doi: 10.7508/ibj.2015.04.001. Epub 2015 Jul 26.

Expression Analysis of mir-21 and mir-221 in Cancerous Tissues from Iranian Patients with Gastric Cancer

Hosein Effatpanah  1   2 , Reza Yadegarazari  1   2 , Manoochehr Karami  3 , Amir Majlesi  4 , Nooshin Shabab  1   2 , Massoud Saidijam  1   2
Affiliations

Expression Analysis of mir-21 and mir-221 in Cancerous Tissues from Iranian Patients with Gastric Cancer

Hosein Effatpanah et al. Iran Biomed J. 2015.

Abstract

Background: Early detection is a key to survival for gastric cancer. Molecular markers such as miRNA (microRNA) can have great importance in the early diagnosis of gastric cancer. Expression of miR-21 and miR-221 are deregulated in many types of human cancers. This study aimed to investigate the differences in miRNA expression patterns within the Iranian population.

Methods: Total RNA was extracted from gastric cancer tissues and adjacent non-cancerous tissues from 32 patients. Expression levels of miR-21 and miR-221 were detected by Real time RT-PCR using a specific primer, with 5s rRNA as the internal reference gene.

Results: Our data showed that the expression levels of miR-21 and miR-221 in gastric cancer samples were significantly higher than in paired non-cancerous samples (P value less than 0.05). The receiver operating characteristic (ROC) analyses yielded the area under the curve (AUC) values of 80.30 for miR-21 and 93.30 for miR-221, and combined ROC analysis revealed the highest AUC value of 96.90 in discriminating GC patients from healthy controls.

Conclusion: It seems that miR-21 and miR-221 expression pattern in Iranian patients with gastric cancer are similar to any other population. Considering the increased expression level of two miRNAs in cancerous tissue compared to normal tissue as well as the area under ROC curve, miR-21 and miR-221 can be used for early detection of gastric cancer.

Keywords: MicroRNAs; Stomach neoplasms; Tumor markers.

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Figures

Fig. 1
Fig. 1
Ct values of miR-21 in human gastric tumor tissue and adjacent non-tumor tissue. The ΔCt range was from 0.88 to 6.30 in cancerous tissues, while it was from 3.93 to 8.42 in non-tumor tissues. Black and gray bars represent the expression level of miR-21 in patients and controls, respectively. Number code represents the number of patients
Fig. 2
Fig. 2
Fold change of the expression of the two miRNAs. The mean fold changes of miR-21 and miR-221 were 2.39 ± 2.56 and 7.5 ± 2.48, respectively
Fig. 3
Fig. 3
Ct values of miR-221 in human gastric tumor tissue and adjacent non-tumor tissue. The ΔCt range was from 1.67 to 9.81 in cancerous tissues, while it was from 7.16 to 12.40 in non-tumor tissues. Black and gray bars represent the expression level of miR-221 in patients and controls, respectively. Number code represents the number of patients
Fig. 4
Fig. 4
ROC curve analysis. ROC curve shows AUCs of miR-21 and miR-221 (A) as well as the combination of two miRs (B). The green and blue lines represent miR-21 and miR-221, respectively
See this image and copyright information in PMC

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