Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Sep;12(9):827-30.
doi: 10.1038/nmeth.3481. Epub 2015 Jul 27.

Ultrafast, temporally stochastic STED nanoscopy of millisecond dynamics

Jale Schneider  1   2 , Jasmin Zahn  1   2 , Marta Maglione  3 , Stephan J Sigrist  3 , Jonas Marquard  1   2 , Jakub Chojnacki  4 , Hans-Georg Kräusslich  4 , Steffen J Sahl  5 , Johann Engelhardt  1   2 , Stefan W Hell  1   2   5
Affiliations

Ultrafast, temporally stochastic STED nanoscopy of millisecond dynamics

Jale Schneider et al. Nat Methods. 2015 Sep.

Abstract

Electro-optical scanning (>1,000 frames/s) with pixel dwell times on the order of the lifetime of the fluorescent molecular state renders stimulated emission depletion (STED) nanoscopy temporally stochastic. Photon detection from a molecule occurs stochastically in one of several scanning frames, and the spatial origin of the photon is known with subdiffraction precision. Images are built up by binning consecutive frames, making the time resolution freely adjustable. We demonstrated nanoscopy of vesicle motions in living Drosophila larvae and the cellular uptake of viral particles with 5- to 10-ms temporal resolution.

PubMed Disclaimer

References

    1. J Microsc. 2015 Jan;257(1):31-8 - PubMed
    1. Science. 2008 Apr 11;320(5873):246-9 - PubMed
    1. Nat Methods. 2008 Nov;5(11):943-5 - PubMed
    1. Nat Methods. 2013 Jul;10(7):653-8 - PubMed
    1. Anal Chem. 1998 Jul 1;70(13):2651-9 - PubMed

Publication types

MeSH terms

LinkOut - more resources