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. 2015 Jun;12(2):55-9.
doi: 10.14245/kjs.2015.12.2.55. Epub 2015 Jun 30.

Bone Mineral Density Changes after Orchiectomy using a Scrotal Approach in Rats

Seong Jun Ryu  1 , Dal Sung Ryu  1 , Jong Yul Kim  1 , Jeong Yoon Park  1 , Kyung Hyun Kim  1 , Dong Kyu Chin  1 , Keun Su Kim  1 , Yong Eun Cho  1 , Sung Uk Kuh  1
Affiliations

Bone Mineral Density Changes after Orchiectomy using a Scrotal Approach in Rats

Seong Jun Ryu et al. Korean J Spine. 2015 Jun.

Abstract

Objective: To investigate a suitable animal model for studies of male osteoporosis. Osteoporosis has a particularly high incidence in postmenopausal women, resulting in a substantial amount of research with respect to this disease in women. However, research on osteoporosis in men is still lacking.

Methods: Twenty 10-week-old male Sprague Dawley rats were used in this study, including 4 rats used to establish a baseline bone mineral density (BMD). The other 16 rats were divided into two groups: a sham surgery group (n=8), which underwent a sham operation, and an orchiectomized rat group (OCX) (n=8), which underwent bilateral OCX at 10 weeks of age. Bone mineral density was measured in 4 rats from both the sham surgery group and the OCX group 8 weeks after the surgery, while BMD in the remainder of the rats was measured 10 weeks post-surgery.

Results: Femoral BMD at 8 weeks post-surgery was found to be significantly lower in the OCX group compared to the sham group; a finding that was also similar 10 weeks post-surgery.

Conclusion: 8 weeks after undergoing orchiectomy performed via a scrotal, white rats are a suitable model for studies of male osteoporosis.

Keywords: Animal model; Bone mineral density; Femur; Male; Orchiectomy; Osteoporosis.

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Figures

Fig. 1
Fig. 1. BMD measurement using NRecon software. BMD was measured in the femora using micro computed tomography: SkyScan 1173 (Bruker-CT, Kartuizersweg 3B 2550 Kontich, Belgium) and NRecon (Ver. 1.6.9.4) software.
Fig. 2
Fig. 2. Procedures for orchiectomy in rat. Anesthetized rat was laid supine on the operating table. Scrotal shaving was done (A). Betadine prep was performed as an aseptic maneuver (B). A small, 1.0-cm median incision was made through the skin at the tip of the scrotum(C). The cremaster muscles were opened with a small 7-mm incision. Thick black arrow: cremaster muscle. The testicular fat pad was localized and pulled through the incision using blunt forceps. The cauda epididymis was pulled out along with the testis, followed by the caput epididymis, the vas deferens and the testicular blood vessels (D). A single ligature was placed around the vas deferens and blood vessels (E). The testis was then removed. This procedure was repeated for the other testis. Muscle and skin were sutured layer by layer (F). Thick white arrow: Testis and epididymis surrounded by fat pad were totally removed.
Fig. 3
Fig. 3. Micro CT Axial images of femora. (A, C) shows Sham group rats, each rats are POD 8 weeks and POD 10 weeks. (B, D) shows OCX group rats, each rats are 8 weeks and 10 weeks.
Fig. 4
Fig. 4. The changes in BMD(g/cm3) in the Sham and OCX groups. The measured BMD was lowest at 8 weeks, after which BMD increased.
See this image and copyright information in PMC

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