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. 2014 Nov 6:1:79-84.
doi: 10.1016/j.dib.2014.10.003. eCollection 2014 Dec.

Data in support of peptidomic analysis of spermatozoa during epididymal maturation

Valérie Labas  1 , Lucie Spina  1 , Clémence Belleannee  2 , Ana-Paula Teixeira-Gomes  3 , Audrey Gargaros  1 , Françoise Dacheux  2 , Jean-Louis Dacheux  2
Affiliations

Data in support of peptidomic analysis of spermatozoa during epididymal maturation

Valérie Labas et al. Data Brief. .

Abstract

The final differentiation of the male germ cell occurs in the epididymal duct where the spermatozoa develop the ability to be motile and fertilize an ovum. Understanding of these biological processes is the key to understanding and controlling male fertility. Comparative studies between several epididymal maturation states could be an informative approach to finding sperm modifications related to maturation and fertility. Here we show the data from differential peptidomic/proteomic analyses on spermatozoa isolated from 4 epididymal regions (immature to mature stage) using a profiling approach based on MALDI-TOF mass spectrometry and, combined to top-down MS in order to identify peptidoforms and proteoforms. By this way, 172m/z peaks ranging between 2 and 20 kDa were found to be modified during maturation of sperm. A total of 62m/z were identified corresponding to 32 different molecular species. The interpretation of these data can be found in the research article published by Labas and colleagues in the Journal of Proteomics in 2014 [1].

Keywords: Epididymis; Maturation; Peptidome; Spermatozoa.

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Figures

Fig. 1
Fig. 1
Experimental design.
Fig. 2
Fig. 2
Classification of identified molecular species based on biological process and molecular function using PANTHER classification system.
See this image and copyright information in PMC

References

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