Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 May 15;8(5):6866-77.
eCollection 2015.

The effect of PDIA3 gene knockout on the mucosal immune function in IBS rats

Zhao-Meng Zhuang  1 , Xiao-Teng Wang  1 , Lu Zhang  1 , Li-Yuan Tao  1 , Bin Lv  1
Affiliations

The effect of PDIA3 gene knockout on the mucosal immune function in IBS rats

Zhao-Meng Zhuang et al. Int J Clin Exp Med. .

Abstract

Objective: To observe the changes of intestinal inflammation on PDIA3 gene knockout IBS rats and its effect on immune function.

Methods: 36 SD rats were randomly divided into four groups: the control group (n = 8); IBS- empty virus group (IBS-GFP, which); IBS-PDIA3 knockout group (n = 12); IBS- the control group (n = 12). After modeling, colon and ileocecal tissue pathology in each group were observed separately. Changes of immune and inflammatory markers were measured. At the same time, ultrastructural changes in each group were observed by electron microscopy.

Results: Compared with the IBS control group, inflammation was reduced significantly in IBS-PDIA3 knockout group. IgE, IL-4 and IL-9 and the level of intestinal trypsin type were decreased significantly. Furthermore, mast cell degranulation and PAR 2 receptor reduced significantly.

Conclusion: PDIA3 may play an important role in the development of IBS by mediating through immune responses of mucosal abnormalities. However, the mechanism needs to be confirmed in further study.

Keywords: PDIA3; colon; immune response; irritable bowel syndrome; knockout.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Comparisos on morphological of rats’ colon and ileocecal. A: IBS model of the second day, colon tissue 100×; B: IBS model of the second day, ileocecal 100×; C: IBS model of the seventh day, colon tissue 100×; D: IBS model of the seventh day, the ileocecal 100×; E: Normal SD rat’s colon tissue 100×; F: Normal SD rat’s ileocecal 100×.
Figure 2
Figure 2
Quantitative PCR for detection the content of IL-4 and IL-9 mRNA.
Figure 3
Figure 3
Observation of distribution of ileocecal mucosa DC in IBS rats labeled with immunohistochemical CD103 (×400).
Figure 4
Figure 4
Toluidine blue staining method for counting the intestinal mucosa MC (×100).
Figure 5
Figure 5
Observation of morphology of intestinal mucosal mast cell and ultrastructure of degranulation mast cell by TEM.
Figure 6
Figure 6
Expression of PAR-2, PAID3 protein. 1: showed the ileocecal intestinal mucosa in the control group; 2: showed the ileocecal intestinal mucosa in IBS1-GFP group; 3: showed the ileocecal intestinal mucosa in IBS-PAID knockout group; 4: showed ileocecal intestinal mucosa in IBS-control group; 5: showed the colon tissue of the blank control group; 6 showed the colon tissue in IBS1-PAID group. A: *P<0.05, compared to IBS-control group; B: *P<0.05, compared to IBS1-PAID group.
See this image and copyright information in PMC

References

    1. Spinelli A. Irritable bowel syndrome. Clin Drug Investig. 2007;27:15–33. - PubMed
    1. Chang FY, Lu CL, Chen TS. The current prevalence of irritable bowel syndrome in Asia. J Neurogastroenterol Motil. 2010;16:389–400. - PMC - PubMed
    1. Spiegel BM. The burden of IBS: looking at metrics. Curr Gastroenterol Rep. 2009;11:265–269. - PubMed
    1. Ghaith O, El-Halabi M, Hashash JG, Sharara AI. Investigational agents for the irritable bowel syndrome. Expert Opin Investig Drugs. 2010;19:1161–1178. - PubMed
    1. Cremon C, Gargano L, Morselli-Labate AM, Santini D, Cogliandro RF, De Giorgio R, Stanghellini V, Corinaldesi R, Barbara G. Mucosal immune activation in irritable bowel syndrome: gender-dependence and association with digestive symptoms. Am J Gastroenterol. 2009;104:392–400. - PubMed

LinkOut - more resources