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. 2015 May-Jun;23(3):288-94.
doi: 10.1590/1678-775720140458.

Comparison of salivary levels of mucin and amylase and their relation with clinical parameters obtained from patients with aggressive and chronic periodontal disease

Andrea Beatriz Acquier  1 , Alejandra Karina De Couto Pita  1 , Lucila Busch  1 , Gabriel Antonio Sánchez  2
Affiliations

Comparison of salivary levels of mucin and amylase and their relation with clinical parameters obtained from patients with aggressive and chronic periodontal disease

Andrea Beatriz Acquier et al. J Appl Oral Sci. 2015 May-Jun.

Abstract

Objective: Salivary mucin and amylase levels are increased in patients with chronic periodontitis (CP). Due to the fact that aggressive periodontitis (AgP) not only differs from chronic periodontitis in terms of its clinical manifestation, the aim of this study was to compare salivary mucin and amylase levels and their relation to the clinical parameters of patients with aggressive periodontitis with that of patients with chronic periodontitis.

Material and methods: Eighty subjects were divided into two groups: 20 patients with AgP and their 20 matched controls and 20 patients with CP and their 20 matched controls, based on clinical attachment loss (CAL), probing pocket depth (PPD) and bleeding on probing (BOP). Whole unstimulated saliva was obtained and mucin, amylase and protein were determined by colorimetric methods. Pearson's correlation analysis was used to determine the relationship between salivary mucin, amylase and protein levels and the clinical parameters.

Results: Salivary mucin, amylase and protein levels were increased in patients with AgP and CP but there were no differences between them or between control groups. Pearson's correlation analysis, determined in the entire subjects studied, showed a positive and significant correlation of mucin, amylase and proteins with CAL and PPD and a negative correlation with the flow rate. When Pearson's correlation analysis was carried out in each group separately, Fisher's z transformation showed no significant difference between both groups.

Conclusion: Comparison of the salivary levels of mucin, amylase and protein and their relationship with clinical parameters of AgP patients with that of CP patients revealed no differences between both groups.

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Figures

Figure 1
Figure 1. Salivary levels of mucin (A), amylase (B) and proteins (C) in unstimulated saliva from patients with aggressive and chronic periodontal disease and their matched controls
Figure 2
Figure 2. Pearson’s correlation analysis between mucin (A), amylase (B) and proteins (C) and clinical attachment level (CAL) in the entire subjects studied
Figure 3
Figure 3. Pearson’s correlation analysis between mucin (A), amylase (B) and proteins (C) and probing pocket depth (PPD) in the entire subjects studied
Figure 4
Figure 4. Pearson’s correlation analysis between mucin (A), amylase (B) and proteins (C) and salivary unstimulated flow rate (UFR) in the entire subjects studied
Figure 5
Figure 5. Pearson’s correlation analysis between mucin and clinical attachment level (CAL) (A and D), probing pocket depth (PPD) (B and E) and unstimulated flow rate (UFR) (C and F) in aggressive periodontitis (AgP) (upper panel) and chronic periodontitis (CP) (lower panel) groups
Figure 6
Figure 6. Pearson’s correlation analysis between amylase and clinical attachment level (CAL) (A and D), probing pocket depth (PPD) (B and E) and unstimulated flow rate (UFR) (C and F) in aggressive periodontitis (AgP) (upper panel) and chronic periodontitis (CP) (lower panel) groups
Figure 7
Figure 7. Pearson’s correlation analysis between total proteins and clinical attachment level (CAL) (A and D), probing pocket depth (PPD) (B and E) and unstimulated flow rate (UFR) (C and F) in aggressive periodontitis (AgP) (upper panel) and chronic periodontitis (CP) (lower panel) groups
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