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. 2015 Sep;8(5):874-82.
doi: 10.1111/1751-7915.12304. Epub 2015 Jul 31.

Butyric acid fermentation from pretreated and hydrolysed wheat straw by an adapted Clostridium tyrobutyricum strain

G N Baroi  1 , I Baumann  1 , P Westermann  1 , H N Gavala  1
Affiliations

Butyric acid fermentation from pretreated and hydrolysed wheat straw by an adapted Clostridium tyrobutyricum strain

G N Baroi et al. Microb Biotechnol. 2015 Sep.

Abstract

Butyric acid is a valuable building-block for the production of chemicals and materials and nowadays it is produced exclusively from petroleum. The aim of this study was to develop a suitable and robust strain of Clostridium tyrobutyricum that produces butyric acid at a high yield and selectivity from lignocellulosic biomasses. Pretreated (by wet explosion) and enzymatically hydrolysed wheat straw (PHWS), rich in C6 and C5 sugars (71.6 and 55.4 g l(-1) of glucose and xylose respectively), was used as substrate. After one year of serial selections, an adapted strain of C. tyrobutyricum was developed. The adapted strain was able to grow in 80% (v v(-1) ) PHWS without addition of yeast extract compared with an initial tolerance to less than 10% PHWS and was able to ferment both glucose and xylose. It is noticeable that the adapted C. tyrobutyricum strain was characterized by a high yield and selectivity to butyric acid. Specifically, the butyric acid yield at 60-80% PHWS lie between 0.37 and 0.46 g g(-1) of sugar, while the selectivity for butyric acid was as high as 0.9-1.0 g g(-1) of acid. Moreover, the strain exhibited a robust response in regards to growth and product profile at pH 6 and 7.

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Figures

Figure 1
Figure 1
Butyric and acetic acid yields and sugar consumption obtained with increasing concentrations of PHWS during the 2nd adaptation phase of C. tyrobutyricum.
Figure 2
Figure 2
Glucose and xylose utilization during batch fermentations of 60% and 70% PHWS by adapted C. tyrobutyricum.
Figure 3
Figure 3
Sugar consumption and acid production profiles during batch fermentation with two consecutive pulse feedings of PHWS.
Figure 4
Figure 4
Yields of butyric and acetic acids and selectivity of butyric acid under different pH values.
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