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Published Erratum
. 2015 Jul 31;10(7):e0134818.
doi: 10.1371/journal.pone.0134818. eCollection 2015.

Correction: The TAL Effector PthA4 Interacts with Nuclear Factors Involved in RNA-Dependent Processes Including a HMG Protein That Selectively Binds Poly(U) RNA

Tiago Antonio de Souza, Adriana Santos Soprano, Nayara Patricia Vieira de Lira, Alexandre José Christino Quaresma, Bianca Alves Pauletti, Adriana Franco Paes Leme, Celso Eduardo Benedetti
Published Erratum

Correction: The TAL Effector PthA4 Interacts with Nuclear Factors Involved in RNA-Dependent Processes Including a HMG Protein That Selectively Binds Poly(U) RNA

Tiago Antonio de Souza et al. PLoS One. .
No abstract available

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Figures

Fig 1
Fig 1. Protein-protein interactions between PthAs and citrus nuclear proteins.
(A) Citrus preys fused to yeast GAL4-AD (GAL4AD-prey) or control plasmid (GAL4AD) were moved into yeast cells carrying one of the four PthA variants fused to GAL4-BD domain as shown in the diagram (1 to 4, respectively). Yeast double-transformants were grown on SC-Trp-Leu-His-Ade in the presence of 5 mM of 3AT. None of prey fusions transactivated the reporter genes when co-transformed with empty bait vector (5). The PthA baits also did not transactivate the reporter genes when co-transformed with the empty prey vector in the same growth conditions (GAL4AD). (B) Western blot detection of eluted fractions from GST pulldown assays using the purified 6xHis-PthAs 1–4 as prey and immobilized GST or GST-fusion proteins as baits. Arrows indicate bands corresponding to the expected size for the GST-fusion proteins CsHMG (~45 kDa), CsTRAX (~55 kDa), CsSMC (~45 kDa), CsRRPMP1 (~50 kDa), CsRRMP2 (~46 kDa), CsPABP1 (~53 kDa) and CsVIP2 (~85 kDa) detected by the GST anti-serum. PthA proteins (~116–122 kDa) were detected using the anti-PthA serum. Recombinant PthAs 3 and 4 were added as references in the first lanes of the gels shown in the middle and right panels, respectively.
See this image and copyright information in PMC

Erratum for

References

    1. de Souza TA, Soprano AS, Lira NPVd, Quaresma AJC, Pauletti BA, Leme AFP, et al. (2012) The TAL Effector PthA4 Interacts with Nuclear Factors Involved in RNA-Dependent Processes Including a HMG Protein That Selectively Binds Poly(U) RNA. PLoS ONE 7(2): e32305 doi:10.1371/journal.pone.0032305 - DOI - PMC - PubMed

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