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. 2015 Oct;83(2):162-4.
doi: 10.1016/j.diagmicrobio.2015.07.002. Epub 2015 Jul 9.

Incubation time of Mycobacterium tuberculosis complex sputum cultures in BACTEC MGIT 960: 4weeks of negative culture is enough for physicians to consider alternative diagnoses

Sam Ogwang  1 , Paul Mubiri  2 , Charles M Bark  3 , Moses L Joloba  4 , W Henry Boom  5 , John L Johnson  5
Affiliations

Incubation time of Mycobacterium tuberculosis complex sputum cultures in BACTEC MGIT 960: 4weeks of negative culture is enough for physicians to consider alternative diagnoses

Sam Ogwang et al. Diagn Microbiol Infect Dis. 2015 Oct.

Abstract

We retrospectively analyzed time to detection of 3747 positive MGIT sputum cultures at a laboratory in a country with heavy burden of tuberculosis. Ninety-nine percent of diagnostic cultures turned positive within 28days, suggesting that physicians may consider alternative diagnoses if sputum cultures remain negative after 4weeks of incubation.

Keywords: MGIT 960; MTB complex; Sputum culture; TTD.

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Figures

Figure 1
Figure 1
Distribution of TTD for 3747 MTB complex-positive sputum cultures
See this image and copyright information in PMC

References

    1. Boehme CC, Nabeta P, Hillemann D, Nicol MP, Shenai S, Krapp F, Allen J, Tahirli R, Blakemore R, Rustomjee R, Milovic A, Jones M, O'Brien SM, Persing DH, Ruesch-Gerdes S, Gotuzzo E, Rodrigues C, Alland D, Perkins MD. Rapid molecular detection of tuberculosis and rifampin resistance. N Engl J Med. 2010;363(11):1005–1015. - PMC - PubMed
    1. Franco-Alvarez de Luna F, Ruiz P, Gutierrez J, Casal M. Evaluation of the GenoType Mycobacteria Direct assay for detection of Mycobacterium tuberculosis complex and four atypical mycobacterial species in clinical samples. J Clin Microbiol. 2006;44(8):3025–3027. - PMC - PubMed
    1. Siddiqi SH, Rusch-Gerdes S. MGIT Procedure Manual for BACTEC MGIT 960 TB System. Foundation for Innovative New Diagnostics; Geneva, Switzerland: 2006.
    1. CLSI . Laboratory detection and identification of mycobacteria; approved guideline. Clinical and Laboratory Standards Institute; Wayne, PA: 2008.
    1. Guler M, Unsal E, Dursun B, Aydln O, Capan N. Factors influencing sputum smear and culture conversion time among patients with new case pulmonary tuberculosis. Int J Clin Pract. 2007;61(2):231–235. - PubMed

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