Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Aug 4;10(8):e0134512.
doi: 10.1371/journal.pone.0134512. eCollection 2015.

Lysis to Kill: Evaluation of the Lytic Abilities, and Genomics of Nine Bacteriophages Infective for Gordonia spp. and Their Potential Use in Activated Sludge Foam Biocontrol

Zoe A Dyson  1 , Joseph Tucci  1 , Robert J Seviour  2 , Steve Petrovski  2
Affiliations

Lysis to Kill: Evaluation of the Lytic Abilities, and Genomics of Nine Bacteriophages Infective for Gordonia spp. and Their Potential Use in Activated Sludge Foam Biocontrol

Zoe A Dyson et al. PLoS One. .

Abstract

Nine bacteriophages (phages) infective for members of the genus Gordonia were isolated from wastewater and other natural water environments using standard enrichment techniques. The majority were broad host range phages targeting more than one Gordonia species. When their genomes were sequenced, they all emerged as double stranded DNA Siphoviridae phages, ranging from 17,562 to 103,424 bp in size, and containing between 27 and 127 genes, many of which were detailed for the first time. Many of these phage genomes diverged from the expected modular genome architecture of other characterized Siphoviridae phages and contained unusual lysis gene arrangements. Whole genome sequencing also revealed that infection with lytic phages does not appear to prevent spontaneous prophage induction in Gordonia malaquae lysogen strain BEN700. TEM sample preparation techniques were developed to view both attachment and replication stages of phage infection.

PubMed Disclaimer

Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. TEM Gordonia phage morphologies.
(A) Phage GMA4 (B) Phage GRU3 (C) Phage GTE8 (D) Phage GMA7 (E) Phage GMA2 (F) Phage GTE6 (G) Phage GMA6 (H) Phage GMA5. Scale = 50 nm.
Fig 2
Fig 2. Stages in Gordonia phage infection cycles.
(A) Attachment stage of phage infection cycle between phage GMA6 and host Gordonia malaquae strain CON67. Scale = 200 nm. (B) Replication of phage GTE6 inside G. terrae strain CON34 cells prior to cell lysis. Arrows indicate phage replicated inside bacterial cells. Scale = 200 nm.
Fig 3
Fig 3. Genome map of nine Gordonia phages.
Grey indicates structural genes identified with mass spectroscopy data.
See this image and copyright information in PMC

References

    1. de los Reyes FL III. 2010. Foaming p 215–258. In Seviour RJ, Nielsen PH (ed), Microbial Ecology of Activated Sludge. IWA Publishing, London.
    1. Drzyzga O. The strengths and weaknesses of Gordonia: a review of an emerging genus with increasing biotechnological potential. Crit Rev Microbiol. 2012; 38(4):300–16. 10.3109/1040841X.2012.668134 - DOI - PubMed
    1. Arenskötter M, Bröker D, Steinbüchel A. Biology of the Metabolically Diverse Genus Gordonia. App Environ Microbiol. 2004; 70:3195–204. - PMC - PubMed
    1. Klatte S, Rainey FA, Kroppenstedt RM. Transfer of Rhodococcus aichiensis Tsukamura 1982 and Nocardia amarae Lechevalier and Lechevalier 1974 to the Genus Gordonia as Gordona aichiensis comb. nov. and Gordona amarae comb. nov. Int J Syst Bacteriol. 1994; 44(4):769–73. - PubMed
    1. Lechevalier MP, Lechevalier HA. Nocardia amarae sp. nov., an Actinomycete Common in Foaming Activated Sludge. Nati J Syst Bacteriol. 1974; 24(2):278–88.

Publication types

LinkOut - more resources