Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Sep;89(1-2):127-41.
doi: 10.1007/s11103-015-0358-2. Epub 2015 Aug 7.

Alterations of histone modifications at the senescence-associated gene HvS40 in barley during senescence

Nicole Ay  1 , Bianka Janack  1 , Andreas Fischer  2 , Gunter Reuter  2 , Klaus Humbeck  3
Affiliations

Alterations of histone modifications at the senescence-associated gene HvS40 in barley during senescence

Nicole Ay et al. Plant Mol Biol. 2015 Sep.

Abstract

The barley gene HvS40, encoding a putative regulator of leaf senescence, is strongly induced during leaf senescence. As shown by chromatin immunoprecipitation, euchromatic histone modification H3K9ac is added at promoter close to ATG and coding sequence of HvS40 after onset of senescence. In parallel, level of heterochromatic H3K9me2 decreases at this gene. Bisulfite sequencing revealed no DNA-methylation in this region, but a heavily methylated DNA-island, starting 664 bp upstream from translational start site in both, mature and senescent leaves. A decrease in DNA methylation in senescing leaves could be shown at one specific CpG motif at the end of this methylation island. In addition, global changes in chromatin structure during senescence were analyzed via immunocytology, revealing senescence-associated changes in spatial distribution of heterochromatic H3K9me2 patterns in the nuclei. Our results prove a senescence-specific mechanism, altering histone modification marks at distinct sequences of the senescence-associated gene HvS40 and altering distribution of heterochromatic areas in the nuclei.

Keywords: DNA methylation; Epigenetic; Histone modifications; Hordeum vulgare; HvS40; Leaf senescence.

PubMed Disclaimer

References

    1. Chromosome Res. 2003;11(3):277-304 - PubMed
    1. Plant J. 2003 Mar;33(6):967-73 - PubMed
    1. Curr Opin Plant Biol. 2003 Feb;6(1):20-8 - PubMed
    1. Proc Natl Acad Sci U S A. 2010 Oct 26;107(43):18729-34 - PubMed
    1. Plant Cell Physiol. 2012 May;53(5):847-56 - PubMed

Publication types

LinkOut - more resources