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. 1989 Dec 29:497:131-7.
doi: 10.1016/0378-4347(89)80012-x.

Measurement of K vitamins in animal tissues by high-performance liquid chromatography with fluorimetric detection

K Hirauchi  1 , T Sakano, S Notsumoto, T Nagaoka, A Morimoto, K Fujimoto, S Masuda, Y Suzuki
Affiliations

Measurement of K vitamins in animal tissues by high-performance liquid chromatography with fluorimetric detection

K Hirauchi et al. J Chromatogr. .

Abstract

A highly sensitive method for measuring endogenous phylloquinone and menaquinones in animal tissues was developed, based on high-performance liquid chromatography with coulometric reduction and fluorimetric detection, following extraction from tissue homogenate and purification on a Sep-Pak silica cartridge followed by thin-layer chromatography. The detection limits of phylloquinone, menaquinone-4, -6, -10 and -13 were 40, 40, 50, 70 and 80 pg/g in rat liver, respectively.

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