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. 1979 Nov;28(2):281-91.
doi: 10.1016/S0006-3495(79)85176-0.

Fluorescence redistribution after photobleaching. A new multipoint analysis of membrane translational dynamics

D E Koppel

Fluorescence redistribution after photobleaching. A new multipoint analysis of membrane translational dynamics

D E Koppel. Biophys J. 1979 Nov.

Abstract

A theoretical formulation and experimental methodology are presented for a new multipoint analysis of membrane translational dynamics. The redistribution of fluorescent probe after a localized photobleaching pulse is monitored at several locations by a focused laser beam sequentially scanned through the bleached area. The spatial information so obtained provides a unique sensitivity to possible systematic flow and a direct internal calibration of the characteristic transport distance. These capabilities are demonstrated with experimental data on a reconstituted multibilayer system.

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References

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