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. 2015 Jun 27:7:67-71.
doi: 10.1016/j.nmni.2015.06.007. eCollection 2015 Sep.

MUS-2, a novel variant of the chromosome-encoded β-lactamase MUS-1, from Myroides odoratimimus

C Al-Bayssari  1 , S Kumar Gupta  2 , F Dabboussi  3 , M Hamze  3 , J-M Rolain  2
Affiliations

MUS-2, a novel variant of the chromosome-encoded β-lactamase MUS-1, from Myroides odoratimimus

C Al-Bayssari et al. New Microbes New Infect. .

Abstract

The aim of the present study was to investigate the molecular mechanism of carbapenem resistance of three imipenem-resistant isolates of Myroides odoratimimus recovered from two livestock farms of cows and pigeons by rectal swab in Lebanon in January 2014. Investigation of imipenem resistance of these isolates using the modified Hodge test, the EDTA test, the modified CarbaNP test and the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Ultraflex assay showed a carbapenemase activity due to the presence of a chromosome-encoded β-lactamase MUS, verified by PCR. However amplification and sequencing of this chromosomal gene showed a novel variant of it designated MUS-2 by the curators of the Lahey database of β-lactamases (http://www.lahey.org/Studies/webt.asp). Cloning of the bla MUS-2 was performed, followed by protein expression in Escherichia coli TOP 10. Pulsed-field gel electrophoresis clearly showed that the three isolates belonged to the same clone. This study reports a novel variant of the chromosome-encoded bla MUS-1 associated with carbapenem resistance in Myroides odoratimimus and shows that animals may represent a reservoir of bacteria harbouring several variants of resistance genes.

Keywords: Animals; Myroides odoratimimus; blaMUS-2; carbapenem-resistance; metallo-β-lactamase.

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Figures

Fig. 1
Fig. 1
Amino acid sequence alignment between the MUS-1 and MUS-2 proteins.
Fig. 2
Fig. 2
Pulsed-field gel electrophoresis of SmaI DNA restriction digests of the three Myroides odoratimimus isolates.
See this image and copyright information in PMC

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